| Summary: | <p>Abstract</p> <p>Background</p> <p><it>Launaea procumbens</it> (LP) has been used as a food supplement in Pakistan. In this study methanolic crude extract (LPME) of the whole plant and its different fractions; n-hexane (LPHE); ethyl acetate (LPEE) and chloroform (LPCE) were studied for the determination of total flavonoid and phenolics contents along with multifaceted <it>in vitro</it> scavenging assays.</p> <p>Results</p> <p>Considerable amount of flavonoid and phenolics contents were found in all the fractions. Methanol and chloroform fraction exhibited efficient scavenging of DPPH·, ABTS·+, ·OH, superoxide, lipid peroxide and nitric oxide free radicals. Significant correlation was found between DPPH·, ABTS·+, superoxide radical, β-carotene bleaching restraint and phosphomolybdenum assay with total flavonoids and phenolics contents. High performance chromatography (HPLC) of LPME revealed the presence of vitexin, orientin, rutin, hyperoside, catechin and myricetin.</p> <p>Conclusion</p> <p>These results reveal the presence of bioactive compounds in LPME, which might be contributed towards the various <it>in vitro</it> scavenging.</p>
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