Antimicrobial Resistance of Staphylococcal Strains Isolated from Various Pathological Products

• Main Authors: Laura-Mihaela SIMON, Lia-Monica JUNIE, Daniela HOMORODEAN
• Format: Article
• Language: English
• Published: Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca 2010-12-01
• Series: Applied Medical Informatics
• Subjects: Resistance; Antibacterial agents; MRSA (methicillin-resistant S. aureus); Staphylococcus.
• Online Access: http://ami.info.umfcluj.ro/Full-text/AMI_27_2010/AMI27%284%29_074_080.pdf

Background: The optimal choice of antimicrobial therapy is an important problem in hospital environment in which the selection of resistant and virulent strains easy occurs. S. aureus and especially MRSA(methicillin-resistant S. aureus) creates difficulties in both treatment and prevention of nosocomial infections. Aim: The purpose of this study is to determine the sensitivity and the resistance to chemotherapy of staphylococci strains isolated from various pathological products. Material and Method: We identified Staphylococccus species after morphological appearance, culture properties, the production of coagulase, hemolisines and the enzyme activity. The susceptibility tests were performed on Mueller-Hinton medium according to CLSI (Clinical and Laboratory Standards Institute). Results: The strains were: MSSA (methicillin-susceptible S. aureus) (74%), MRSA (8%), MLS B (macrolides, lincosamides and type B streptogramines resistance) (12%) and MRSA and MLS B (6%). MRSA strains were more frequently isolated from sputum. MRSA associated with the MLS B strains were more frequently isolated from pus. MLS B strains were more frequently isolated from sputum and throat secretions. All S. aureus strains were susceptible to vancomycin and teicoplanin. Conclusions: All staphylococcal infections require resistance testing before treatment. MLS B shows a high prevalence among strains of S. aureus. The association between MLS B and MRSA remains a major problem in Romania.