Antimicrobial Resistance of Staphylococcal Strains Isolated from Various Pathological Products

Background: The optimal choice of antimicrobial therapy is an important problem in hospital environment in which the selection of resistant and virulent strains easy occurs. S. aureus and especially MRSA(methicillin-resistant S. aureus) creates difficulties in both treatment and prevention of nosoco...

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Bibliographic Details
Main Authors: Laura-Mihaela SIMON, Lia-Monica JUNIE, Daniela HOMORODEAN
Format: Article
Language:English
Published: Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca 2010-12-01
Series:Applied Medical Informatics
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Online Access:http://ami.info.umfcluj.ro/Full-text/AMI_27_2010/AMI27%284%29_074_080.pdf
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Summary:Background: The optimal choice of antimicrobial therapy is an important problem in hospital environment in which the selection of resistant and virulent strains easy occurs. S. aureus and especially MRSA(methicillin-resistant S. aureus) creates difficulties in both treatment and prevention of nosocomial infections. Aim: The purpose of this study is to determine the sensitivity and the resistance to chemotherapy of staphylococci strains isolated from various pathological products. Material and Method: We identified Staphylococccus species after morphological appearance, culture properties, the production of coagulase, hemolisines and the enzyme activity. The susceptibility tests were performed on Mueller-Hinton medium according to CLSI (Clinical and Laboratory Standards Institute). Results: The strains were: MSSA (methicillin-susceptible S. aureus) (74%), MRSA (8%), MLS B (macrolides, lincosamides and type B streptogramines resistance) (12%) and MRSA and MLS B (6%). MRSA strains were more frequently isolated from sputum. MRSA associated with the MLS B strains were more frequently isolated from pus. MLS B strains were more frequently isolated from sputum and throat secretions. All S. aureus strains were susceptible to vancomycin and teicoplanin. Conclusions: All staphylococcal infections require resistance testing before treatment. MLS B shows a high prevalence among strains of S. aureus. The association between MLS B and MRSA remains a major problem in Romania.
ISSN:1224-5593