Molecular and Biological Characterization of an Isolate of Cucumber mosaic virus from Glycine soja by Generating its Infectious Full-genome cDNA Clones

• Main Authors: Mi Sa Vo Phan, Jang-Kyun Seo, Hong-Soo Choi, Su-Heon Lee, Kook-Hyung Kim
• Format: Article
• Language: English
• Published: Hanrimwon Publishing Company 2014-06-01
• Series: The Plant Pathology Journal
• Subjects: Cucumber mosaic virus; sequence analysis; phylogenetic analyses; infectious clones; wild soybean
• Online Access: http://www.ncbi.nlm.nih.gov/pmc/articles/4174851

Molecular and biological characteristics of an isolate of Cucumber mosaic virus (CMV) from Glycine soja (wild soybean), named as CMV-209, was examined in this study. Comparison of nucleotide sequences and phylogenetic analyses of CMV-209 with the other CMV strains revealed that CMV-209 belonged to CMV subgroup I. However, CMV-209 showed some genetic distance from the CMV strains assigned to subgroup IA or subgroup IB. Infectious full-genome cDNA clones of CMV-209 were generated under the control of the Cauliflower mosaic virus 35S promoter. Infectivity of the CMV-209 clones was evaluated in Nicotiana benthamiana and various legume species. Our assays revealed that CMV-209 could systemically infect Glycine soja (wild soybean) and Pisum sativum (pea) as well as N. benthamiana, but not the other legume species.