Ekstrak Etanol Propolis Menurunkan Kadar IL-17 Serum pada Mencit Balb/C Model Asma Kronik

• Main Authors: Sri Hartati Hadinoto, Sarsono, Siti Aisyah, Diding Heri Prasetyo
• Format: Article
• Language: English
• Published: Universitas Padjajaran 2013-12-01
• Series: Majalah Kedokteran Bandung
• Subjects: Asthma; interleukin-17 (IL-17); propolis
• Online Access: http://journal.fk.unpad.ac.id/index.php/mkb/article/view/167

Interleukin-17 (IL-17) induces the neutrophil recruitment into the respiratory tract while playing a role in the pathogenesis of asthma and determining the severity of disease. The aim of this study was to analyze the effect of ethanol extract of propolis (EEP) Lawu mountain isolates on IL-17 serum levels in Balb/C mice model with chronic asthma. This study was an experimental research laboratory with 28 male Balb/C mice divided into control, asthma, asthma+EEP and asthma+antihistamine groups. The study was conducted at the Histology and Biomedical Laboratory, Faculty of Medicine, Sebelas Maret University, Surakarta, during April to September 2012. A model of chronic asthma was induced in male Balb/C mice using an intraperitoneally (i.p.) injection ovalbumin (OVA, 100 μg/mice) and aluminum hydroxide (10%, 50 mg/mice) in phosphate-buffered saline (PBS). Experimental animals were sensitized for 6 weeks with inhaled 2% (w/v) OVA aerosol in 0.9% NaCl for 30 minutes/day, 3 days in a week, for asthma, asthma with EEP, and asthma with antihistamine mice model groups. Control mice were not inoculated during the study. Detection of IL-17 serum level was conducted by using enzyme-linked immunosorbent assay (ELISA). One way analysis of variance (ANOVA) for IL-17 serum level and p<0.05 were used to determine significant differences. Serum levels of IL-17 showed 17.15±8.25 pg/mL in control group and 38.98±7.57 pg/mL in antihistamine, respectively. Ethanol extract of propolis showed significant decreased in IL-17 levels (75.62±17.64 pg/mL vs 45.59±10.69 pg/mL, p<0.001) compared to asthma group. In conclusion, EEP Lawu mountain isolates can decrease IL-17 level in chronic asthma animal models.