Establishment of an Efficient and Flexible Genetic Manipulation Platform Based on a Fosmid Library for Rapid Generation of Recombinant Pseudorabies Virus

Establishment of an Efficient and Flexible Genetic Manipulation Platform Based on a Fosmid Library for Rapid Generation of Recombinant Pseudorabies Virus

Conventional genetic engineering of pseudorabies virus (PRV) is essentially based on homologous recombination or bacterial artificial chromosome. However, these techniques require multiple plaque purification, which is labor-intensive and time-consuming. The aim of the present study was to develop a...

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Bibliographic Details
Main Authors: Mo Zhou, Muhammad Abid, Hang Yin, Hongxia Wu, Teshale Teklue, Hua-Ji Qiu, Yuan Sun
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-09-01
Series:Frontiers in Microbiology
Subjects:
pseudorabies virus
fosmid library
full-length genome assemble
genetic manipulation platform
recombinant PRV
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2018.02132/full

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