Identification of the transcription factor Znc1p, which regulates the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica.

The dimorphic yeast Yarrowia lipolytica is used as a model to study fungal differentiation because it grows as yeast-like cells or forms hyphal cells in response to changes in environmental conditions. Here, we report the isolation and characterization of a gene, ZNC1, involved in the dimorphic tran...

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Main Authors: Azul Martinez-Vazquez, Angelica Gonzalez-Hernandez, Angel Domínguez, Richard Rachubinski, Meritxell Riquelme, Patricia Cuellar-Mata, Juan Carlos Torres Guzman
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23826133/pdf/?tool=EBI
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spelling doaj-209819f435b3475bb34e69516a1519b72021-03-03T23:12:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6679010.1371/journal.pone.0066790Identification of the transcription factor Znc1p, which regulates the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica.Azul Martinez-VazquezAngelica Gonzalez-HernandezAngel DomínguezRichard RachubinskiMeritxell RiquelmePatricia Cuellar-MataJuan Carlos Torres GuzmanThe dimorphic yeast Yarrowia lipolytica is used as a model to study fungal differentiation because it grows as yeast-like cells or forms hyphal cells in response to changes in environmental conditions. Here, we report the isolation and characterization of a gene, ZNC1, involved in the dimorphic transition in Y. lipolytica. The ZNC1 gene encodes a 782 amino acid protein that contains a Zn(II)2C6 fungal-type zinc finger DNA-binding domain and a leucine zipper domain. ZNC1 transcription is elevated during yeast growth and decreases during the formation of mycelium. Cells in which ZNC1 has been deleted show increased hyphal cell formation. Znc1p-GFP localizes to the nucleus, but mutations within the leucine zipper domain of Znc1p, and to a lesser extent within the Zn(II)2C6 domain, result in a mislocalization of Znc1p to the cytoplasm. Microarrays comparing gene expression between znc1::URA3 and wild-type cells during both exponential growth and the induction of the yeast-to-hypha transition revealed 1,214 genes whose expression was changed by 2-fold or more under at least one of the conditions analyzed. Our results suggest that Znc1p acts as a transcription factor repressing hyphal cell formation and functions as part of a complex network regulating mycelial growth in Y. lipolytica.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23826133/pdf/?tool=EBI
collection DOAJ
language English
format Article
sources DOAJ
author Azul Martinez-Vazquez
Angelica Gonzalez-Hernandez
Angel Domínguez
Richard Rachubinski
Meritxell Riquelme
Patricia Cuellar-Mata
Juan Carlos Torres Guzman
spellingShingle Azul Martinez-Vazquez
Angelica Gonzalez-Hernandez
Angel Domínguez
Richard Rachubinski
Meritxell Riquelme
Patricia Cuellar-Mata
Juan Carlos Torres Guzman
Identification of the transcription factor Znc1p, which regulates the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica.
PLoS ONE
author_facet Azul Martinez-Vazquez
Angelica Gonzalez-Hernandez
Angel Domínguez
Richard Rachubinski
Meritxell Riquelme
Patricia Cuellar-Mata
Juan Carlos Torres Guzman
author_sort Azul Martinez-Vazquez
title Identification of the transcription factor Znc1p, which regulates the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica.
title_short Identification of the transcription factor Znc1p, which regulates the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica.
title_full Identification of the transcription factor Znc1p, which regulates the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica.
title_fullStr Identification of the transcription factor Znc1p, which regulates the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica.
title_full_unstemmed Identification of the transcription factor Znc1p, which regulates the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica.
title_sort identification of the transcription factor znc1p, which regulates the yeast-to-hypha transition in the dimorphic yeast yarrowia lipolytica.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2013-01-01
description The dimorphic yeast Yarrowia lipolytica is used as a model to study fungal differentiation because it grows as yeast-like cells or forms hyphal cells in response to changes in environmental conditions. Here, we report the isolation and characterization of a gene, ZNC1, involved in the dimorphic transition in Y. lipolytica. The ZNC1 gene encodes a 782 amino acid protein that contains a Zn(II)2C6 fungal-type zinc finger DNA-binding domain and a leucine zipper domain. ZNC1 transcription is elevated during yeast growth and decreases during the formation of mycelium. Cells in which ZNC1 has been deleted show increased hyphal cell formation. Znc1p-GFP localizes to the nucleus, but mutations within the leucine zipper domain of Znc1p, and to a lesser extent within the Zn(II)2C6 domain, result in a mislocalization of Znc1p to the cytoplasm. Microarrays comparing gene expression between znc1::URA3 and wild-type cells during both exponential growth and the induction of the yeast-to-hypha transition revealed 1,214 genes whose expression was changed by 2-fold or more under at least one of the conditions analyzed. Our results suggest that Znc1p acts as a transcription factor repressing hyphal cell formation and functions as part of a complex network regulating mycelial growth in Y. lipolytica.
url https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23826133/pdf/?tool=EBI
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