The Two Translationally Controlled Tumor Protein Genes, CsTCTP1 and CsTCTP2, Are Negative Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea

The Two Translationally Controlled Tumor Protein Genes, CsTCTP1 and CsTCTP2, Are Negative Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea

Pathogen stress often significantly decreases cucumber production. However, knowledge regarding the molecular mechanism and signals of cucumber disease resistance is far from complete. Here, we report two translationally controlled tumor protein genes, CsTCTP1 and CsTCTP2, that are both negative mod...

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Main Authors: Xiangnan Meng, Yang Yu, Junyue Zhao, Na Cui, Tiefeng Song, Yun Yang, Haiyan Fan
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-04-01
Series:Frontiers in Plant Science
Subjects:
ABA signaling pathway
Cucumis sativus
Sphaerotheca fuliginea
translationally controlled tumor protein
transient transformation
TOR signaling pathway
Online Access:http://journal.frontiersin.org/article/10.3389/fpls.2018.00544/full
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spelling doaj-2084ec11e42a4362afa2ac83d9f228212020-11-24T21:38:18ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-04-01910.3389/fpls.2018.00544354859The Two Translationally Controlled Tumor Protein Genes, CsTCTP1 and CsTCTP2, Are Negative Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuligineaXiangnan Meng0Xiangnan Meng1Yang Yu2Junyue Zhao3Na Cui4Tiefeng Song5Yun Yang6Haiyan Fan7Haiyan Fan8College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang, ChinaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, ChinaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, ChinaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, ChinaVegetable Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang, ChinaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, ChinaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Protected Horticulture of Ministry of Education, Shenyang Agricultural University, Shenyang, ChinaPathogen stress often significantly decreases cucumber production. However, knowledge regarding the molecular mechanism and signals of cucumber disease resistance is far from complete. Here, we report two translationally controlled tumor protein genes, CsTCTP1 and CsTCTP2, that are both negative modulators in the Cucumis sativus defense response to Sphaerotheca fuliginea. Subcellular localization analysis showed that CsTCTP1 and CsTCTP2 were both localized in the cytoplasm. Expression analysis indicated that the transcript levels of CsTCTP1 and CsTCTP2 were linked to the degree of cucumber resistance to S. fuliginea. Transient overexpression of either CsTCTP1 or CsTCTP2 in cucumber cotyledons impaired resistance to S. fuliginea, whereas silencing of either CsTCTP1 or CsTCTP2 enhanced cucumber resistance to S. fuliginea. The relationship of several defense-related genes and ABA and target of rapamycin (TOR) signaling pathway-related genes to the overexpressing and silencing of CsTCTP1/CsTCTP2 in non-infested cucumber plants was investigated. The results indicated that CsTCTP1 participates in the defense response to S. fuliginea by regulating the expression of certain defense-associated genes and/or ABA signaling pathway-associated genes, and CsTCTP2 participates through regulating the expression of TOR signaling pathway-associated genes. Our findings will guide enhancing the resistance of cucumber to powdery mildew.http://journal.frontiersin.org/article/10.3389/fpls.2018.00544/fullABA signaling pathwayCucumis sativusSphaerotheca fuligineatranslationally controlled tumor proteintransient transformationTOR signaling pathway
collection DOAJ
language English
format Article
sources DOAJ
author Xiangnan Meng
Xiangnan Meng
Yang Yu
Junyue Zhao
Na Cui
Tiefeng Song
Yun Yang
Haiyan Fan
Haiyan Fan
spellingShingle Xiangnan Meng
Xiangnan Meng
Yang Yu
Junyue Zhao
Na Cui
Tiefeng Song
Yun Yang
Haiyan Fan
Haiyan Fan
The Two Translationally Controlled Tumor Protein Genes, CsTCTP1 and CsTCTP2, Are Negative Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea
Frontiers in Plant Science
ABA signaling pathway
Cucumis sativus
Sphaerotheca fuliginea
translationally controlled tumor protein
transient transformation
TOR signaling pathway
author_facet Xiangnan Meng
Xiangnan Meng
Yang Yu
Junyue Zhao
Na Cui
Tiefeng Song
Yun Yang
Haiyan Fan
Haiyan Fan
author_sort Xiangnan Meng
title The Two Translationally Controlled Tumor Protein Genes, CsTCTP1 and CsTCTP2, Are Negative Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea
title_short The Two Translationally Controlled Tumor Protein Genes, CsTCTP1 and CsTCTP2, Are Negative Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea
title_full The Two Translationally Controlled Tumor Protein Genes, CsTCTP1 and CsTCTP2, Are Negative Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea
title_fullStr The Two Translationally Controlled Tumor Protein Genes, CsTCTP1 and CsTCTP2, Are Negative Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea
title_full_unstemmed The Two Translationally Controlled Tumor Protein Genes, CsTCTP1 and CsTCTP2, Are Negative Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea
title_sort two translationally controlled tumor protein genes, cstctp1 and cstctp2, are negative modulators in the cucumis sativus defense response to sphaerotheca fuliginea
publisher Frontiers Media S.A.
series Frontiers in Plant Science
issn 1664-462X
publishDate 2018-04-01
description Pathogen stress often significantly decreases cucumber production. However, knowledge regarding the molecular mechanism and signals of cucumber disease resistance is far from complete. Here, we report two translationally controlled tumor protein genes, CsTCTP1 and CsTCTP2, that are both negative modulators in the Cucumis sativus defense response to Sphaerotheca fuliginea. Subcellular localization analysis showed that CsTCTP1 and CsTCTP2 were both localized in the cytoplasm. Expression analysis indicated that the transcript levels of CsTCTP1 and CsTCTP2 were linked to the degree of cucumber resistance to S. fuliginea. Transient overexpression of either CsTCTP1 or CsTCTP2 in cucumber cotyledons impaired resistance to S. fuliginea, whereas silencing of either CsTCTP1 or CsTCTP2 enhanced cucumber resistance to S. fuliginea. The relationship of several defense-related genes and ABA and target of rapamycin (TOR) signaling pathway-related genes to the overexpressing and silencing of CsTCTP1/CsTCTP2 in non-infested cucumber plants was investigated. The results indicated that CsTCTP1 participates in the defense response to S. fuliginea by regulating the expression of certain defense-associated genes and/or ABA signaling pathway-associated genes, and CsTCTP2 participates through regulating the expression of TOR signaling pathway-associated genes. Our findings will guide enhancing the resistance of cucumber to powdery mildew.
topic ABA signaling pathway
Cucumis sativus
Sphaerotheca fuliginea
translationally controlled tumor protein
transient transformation
TOR signaling pathway
url http://journal.frontiersin.org/article/10.3389/fpls.2018.00544/full
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