Facile and Cost-Effective Detection of Saxitoxin Exploiting Aptamer Structural Switching

Facile and Cost-Effective Detection of Saxitoxin Exploiting Aptamer Structural Switching

A simple method to detect saxitoxin (STX), one of the main components of the paralytic shellfish poison from red tide, has been developed. By using a next generation dye for double-stranded DNA we were able to differentiate fluorescence from STX-binding aptamers when exposed to different concentrati...

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Bibliographic Details
Main Authors: Karol Alfaro, Paulina Bustos, Ciara O´Sullivan, Pablo Conejeros
Format: Article
Language:English
Published: University of Zagreb 2015-01-01
Series:Food Technology and Biotechnology
Subjects:
red tide
paralytic shellfish poison
aptamers
saxitoxin
high resolution melting
Online Access:http://hrcak.srce.hr/file/214364
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spelling doaj-207666c34d5947488de917887d10e8cd2020-11-25T03:31:17ZengUniversity of ZagrebFood Technology and Biotechnology1330-98621334-26062015-01-01533337341Facile and Cost-Effective Detection of Saxitoxin Exploiting Aptamer Structural SwitchingKarol Alfaro0Paulina Bustos1Ciara O´Sullivan2Pablo Conejeros3Centro de Investigación y Gestión de Recursos Naturales, Facultad de Ciencias, Universidad de Valparaíso, Valparaíso, ChileCentro de Investigación y Gestión de Recursos Naturales, Facultad de Ciencias, Universidad de Valparaíso, Valparaíso, ChileNanobiotechnology and Bioanalysis Group, Department of Chemical Engineering, Universitat Rovira i Virgili,Tarragona, SpainCentro de Investigación y Gestión de Recursos Naturales, Facultad de Ciencias, Universidad de Valparaíso, Valparaíso, ChileA simple method to detect saxitoxin (STX), one of the main components of the paralytic shellfish poison from red tide, has been developed. By using a next generation dye for double-stranded DNA we were able to differentiate fluorescence from STX-binding aptamers when exposed to different concentrations of STX, suggesting a change in aptamer folding upon target binding. The developed method is extremely rapid, only requiring small sample volumes, with quantitative results in the concentration range of 15 ng/mL to 3 μg/mL of STX, with a detection limit of 7.5 ng/mL.http://hrcak.srce.hr/file/214364red tideparalytic shellfish poisonaptamerssaxitoxinhigh resolution melting
collection DOAJ
language English
format Article
sources DOAJ
author Karol Alfaro
Paulina Bustos
Ciara O´Sullivan
Pablo Conejeros
spellingShingle Karol Alfaro
Paulina Bustos
Ciara O´Sullivan
Pablo Conejeros
Facile and Cost-Effective Detection of Saxitoxin Exploiting Aptamer Structural Switching
Food Technology and Biotechnology
red tide
paralytic shellfish poison
aptamers
saxitoxin
high resolution melting
author_facet Karol Alfaro
Paulina Bustos
Ciara O´Sullivan
Pablo Conejeros
author_sort Karol Alfaro
title Facile and Cost-Effective Detection of Saxitoxin Exploiting Aptamer Structural Switching
title_short Facile and Cost-Effective Detection of Saxitoxin Exploiting Aptamer Structural Switching
title_full Facile and Cost-Effective Detection of Saxitoxin Exploiting Aptamer Structural Switching
title_fullStr Facile and Cost-Effective Detection of Saxitoxin Exploiting Aptamer Structural Switching
title_full_unstemmed Facile and Cost-Effective Detection of Saxitoxin Exploiting Aptamer Structural Switching
title_sort facile and cost-effective detection of saxitoxin exploiting aptamer structural switching
publisher University of Zagreb
series Food Technology and Biotechnology
issn 1330-9862
1334-2606
publishDate 2015-01-01
description A simple method to detect saxitoxin (STX), one of the main components of the paralytic shellfish poison from red tide, has been developed. By using a next generation dye for double-stranded DNA we were able to differentiate fluorescence from STX-binding aptamers when exposed to different concentrations of STX, suggesting a change in aptamer folding upon target binding. The developed method is extremely rapid, only requiring small sample volumes, with quantitative results in the concentration range of 15 ng/mL to 3 μg/mL of STX, with a detection limit of 7.5 ng/mL.
topic red tide
paralytic shellfish poison
aptamers
saxitoxin
high resolution melting
url http://hrcak.srce.hr/file/214364
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AT paulinabustos facileandcosteffectivedetectionofsaxitoxinexploitingaptamerstructuralswitching
AT ciaraosullivan facileandcosteffectivedetectionofsaxitoxinexploitingaptamerstructuralswitching
AT pabloconejeros facileandcosteffectivedetectionofsaxitoxinexploitingaptamerstructuralswitching
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