Facile and Cost-Effective Detection of Saxitoxin Exploiting Aptamer Structural Switching

Facile and Cost-Effective Detection of Saxitoxin Exploiting Aptamer Structural Switching

A simple method to detect saxitoxin (STX), one of the main components of the paralytic shellfish poison from red tide, has been developed. By using a next generation dye for double-stranded DNA we were able to differentiate fluorescence from STX-binding aptamers when exposed to different concentrati...

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Bibliographic Details
Main Authors: Karol Alfaro, Paulina Bustos, Ciara O´Sullivan, Pablo Conejeros
Format: Article
Language:English
Published: University of Zagreb 2015-01-01
Series:Food Technology and Biotechnology
Subjects:
red tide
paralytic shellfish poison
aptamers
saxitoxin
high resolution melting
Online Access:http://hrcak.srce.hr/file/214364
Description
Summary:A simple method to detect saxitoxin (STX), one of the main components of the paralytic shellfish poison from red tide, has been developed. By using a next generation dye for double-stranded DNA we were able to differentiate fluorescence from STX-binding aptamers when exposed to different concentrations of STX, suggesting a change in aptamer folding upon target binding. The developed method is extremely rapid, only requiring small sample volumes, with quantitative results in the concentration range of 15 ng/mL to 3 μg/mL of STX, with a detection limit of 7.5 ng/mL.
ISSN:1330-9862
1334-2606

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