Deposition of Cell Culture Coatings Using a Cold Plasma Deposition Method

Collagen coatings were applied onto polystyrene microplates using a cold atmospheric pressure plasma process. The coatings were compared to standard wet chemical collagen thin films using microscopy, surface energy, infra-red spectroscopy, electrophoresis, and cell culture techniques. Thin films wer...

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Main Authors:	Denis O’Sullivan, Hazel McArdle, Sing Wei Ng, Paula Bourke, Robert Forster, Liam O’Neill
Format:	Article
Language:	English
Published:	MDPI AG 2020-09-01
Series:	Applied Sciences
Subjects:	plasma polymerization corona discharge biomolecule deposition collagen protein voltammetry
Online Access:	https://www.mdpi.com/2076-3417/10/19/6670

id	doaj-20549a33bb2b4207a3633b5b80dcacae
record_format	Article
spelling	doaj-20549a33bb2b4207a3633b5b80dcacae2020-11-25T03:38:21ZengMDPI AGApplied Sciences2076-34172020-09-01106670667010.3390/app10196670Deposition of Cell Culture Coatings Using a Cold Plasma Deposition MethodDenis O’Sullivan0Hazel McArdle1Sing Wei Ng2Paula Bourke3Robert Forster4Liam O’Neill5TheraDep, Questum, Ballingarrane Science and Technology Park, Co. Tipperary, Clonmel, IrelandSchool of Chemical Sciences, FutureNeuro SFI Research Centre, National Centre for Centre Research, Dublin City University, Dublin D9, IrelandSchool of Biosystems and Food Engineering, University College Dublin, Belfield, Dublin D4, IrelandSchool of Biosystems and Food Engineering, University College Dublin, Belfield, Dublin D4, IrelandSchool of Chemical Sciences, FutureNeuro SFI Research Centre, National Centre for Centre Research, Dublin City University, Dublin D9, IrelandTheraDep, Questum, Ballingarrane Science and Technology Park, Co. Tipperary, Clonmel, IrelandCollagen coatings were applied onto polystyrene microplates using a cold atmospheric pressure plasma process. The coatings were compared to standard wet chemical collagen thin films using microscopy, surface energy, infra-red spectroscopy, electrophoresis, and cell culture techniques. Thin films were also deposited on gold electrodes using both coating methods and their structural and barrier properties probed using cyclic voltammetry. While the wet chemical technique produced a thicker deposit, both films appear equivalent in terms of coverage, porosity, structure, and chemistry. Significantly, the cold plasma method preserves both the primary and secondary structure of the protein and this results in high biocompatibility and cell activity that is at least equivalent to the standard wet chemical technique. The significance of these results is discussed in relation to the benefits of a single step plasma coating in comparison to the traditional multi-step aseptic coating technique.https://www.mdpi.com/2076-3417/10/19/6670plasma polymerizationcorona dischargebiomolecule depositioncollagenprotein voltammetry
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Denis O’Sullivan Hazel McArdle Sing Wei Ng Paula Bourke Robert Forster Liam O’Neill
spellingShingle	Denis O’Sullivan Hazel McArdle Sing Wei Ng Paula Bourke Robert Forster Liam O’Neill Deposition of Cell Culture Coatings Using a Cold Plasma Deposition Method Applied Sciences plasma polymerization corona discharge biomolecule deposition collagen protein voltammetry
author_facet	Denis O’Sullivan Hazel McArdle Sing Wei Ng Paula Bourke Robert Forster Liam O’Neill
author_sort	Denis O’Sullivan
title	Deposition of Cell Culture Coatings Using a Cold Plasma Deposition Method
title_short	Deposition of Cell Culture Coatings Using a Cold Plasma Deposition Method
title_full	Deposition of Cell Culture Coatings Using a Cold Plasma Deposition Method
title_fullStr	Deposition of Cell Culture Coatings Using a Cold Plasma Deposition Method
title_full_unstemmed	Deposition of Cell Culture Coatings Using a Cold Plasma Deposition Method
title_sort	deposition of cell culture coatings using a cold plasma deposition method
publisher	MDPI AG
series	Applied Sciences
issn	2076-3417
publishDate	2020-09-01
description	Collagen coatings were applied onto polystyrene microplates using a cold atmospheric pressure plasma process. The coatings were compared to standard wet chemical collagen thin films using microscopy, surface energy, infra-red spectroscopy, electrophoresis, and cell culture techniques. Thin films were also deposited on gold electrodes using both coating methods and their structural and barrier properties probed using cyclic voltammetry. While the wet chemical technique produced a thicker deposit, both films appear equivalent in terms of coverage, porosity, structure, and chemistry. Significantly, the cold plasma method preserves both the primary and secondary structure of the protein and this results in high biocompatibility and cell activity that is at least equivalent to the standard wet chemical technique. The significance of these results is discussed in relation to the benefits of a single step plasma coating in comparison to the traditional multi-step aseptic coating technique.
topic	plasma polymerization corona discharge biomolecule deposition collagen protein voltammetry
url	https://www.mdpi.com/2076-3417/10/19/6670
work_keys_str_mv	AT denisosullivan depositionofcellculturecoatingsusingacoldplasmadepositionmethod AT hazelmcardle depositionofcellculturecoatingsusingacoldplasmadepositionmethod AT singweing depositionofcellculturecoatingsusingacoldplasmadepositionmethod AT paulabourke depositionofcellculturecoatingsusingacoldplasmadepositionmethod AT robertforster depositionofcellculturecoatingsusingacoldplasmadepositionmethod AT liamoneill depositionofcellculturecoatingsusingacoldplasmadepositionmethod
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Deposition of Cell Culture Coatings Using a Cold Plasma Deposition Method

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