An evaluation of immunomagnetic separation-real-time PCR (IMS-RTiPCR) combined assay for rapid and specific detection of Escherichia coli O157:H7 in raw milk and ground beef

An evaluation of immunomagnetic separation-real-time PCR (IMS-RTiPCR) combined assay for rapid and specific detection of Escherichia coli O157:H7 in raw milk and ground beef

Abstract The aim of this study is to optimize a rapid and specific method for detection of E. coli O157:H7 (EHEC) from food samples with high background flora using an immunomagnetic separation-real-time polymerase chain reaction assay (IMS-RTiPCR). For this, EHEC cells were recovered from raw milk...

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Bibliographic Details
Main Authors: Birce MERCANOGLU TABAN, Sait Aykut AYTAC
Format: Article
Language:English
Published: Sociedade Brasileira de Ciência e Tecnologia de Alimentos
Series:Food Science and Technology
Subjects:
Escherichia coli 0157:H7
immunomagnetic separation
milk
raw ground beef
real-time PCR
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019005011102&lng=en&tlng=en

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