Genetic heterogeneity of L-Zagreb mumps virus vaccine strain
<p>Abstract</p> <p>Background</p> <p>The most often used mumps vaccine strains Jeryl Lynn (JL), RIT4385, Urabe-AM9, L-Zagreb and L-3 differ in immunogenicity and reactogenicity. Previous analyses showed that JL, Urabe-AM9 and L-3 are genetically heterogeneous.</p>...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2008-07-01
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| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/5/1/79 |
| Summary: | <p>Abstract</p> <p>Background</p> <p>The most often used mumps vaccine strains Jeryl Lynn (JL), RIT4385, Urabe-AM9, L-Zagreb and L-3 differ in immunogenicity and reactogenicity. Previous analyses showed that JL, Urabe-AM9 and L-3 are genetically heterogeneous.</p> <p>Results</p> <p>We identified the heterogeneity of L-Zagreb throughout the entire genome. Two major variants were defined: variant A being identical to the consensus sequence of viral seeds and vaccine(s) and variant B which differs from variant A in three nucleotide positions. The difference between viral variants in L-Zagreb strain is insufficient for distinct viral strains to be defined. We demonstrated that proportion of variants in L-Zagreb viral population depends on cell substrate used for viral replication in vitro and in vivo.</p> <p>Conclusion</p> <p>L-Zagreb strain should be considered as a single strain composed of at least two variant viral genomes.</p> |
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| ISSN: | 1743-422X |