Expression of TNF, IL1B, and iNOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti-Porphyromonas gingivalis [version 4; peer review: 2 approved]

Expression of TNF, IL1B, and iNOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti-Porphyromonas gingivalis [version 4; peer review: 2 approved]

Porphyromonas gingivalis has virulence factors such as gingipain and lipopolysaccharide, causing bacteremia to reach the brain and activate neuroinflammatory release cytokines. This study analyzed the effect of the co-culture of neuron cells with P. gingivalis coated with anti-P. gingivalis antibodi...

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Main Authors: Endang Winiati Bachtiar, Citra F. Putri, Retno D. Soejoedono, Boy M. Bachtiar
Format: Article
Language:English
Published: F1000 Research Ltd 2021-06-01
Series:F1000Research
Online Access:https://f1000research.com/articles/9-1499/v4
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record_format Article
spelling doaj-1fdd475c8c4c4bcba774529180851e932021-07-26T13:57:24ZengF1000 Research LtdF1000Research2046-14022021-06-01910.12688/f1000research.26749.458087Expression of TNF, IL1B, and iNOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti-Porphyromonas gingivalis [version 4; peer review: 2 approved]Endang Winiati Bachtiar0Citra F. Putri1Retno D. Soejoedono2Boy M. Bachtiar3Department of Oral Biology and Oral Science Research Center, Faculty of Dentistry Universitas Indonesia, Jakarta, DKI, 10430, IndonesiaDepartment of Oral Biology and Oral Science Research Center, Faculty of Dentistry Universitas Indonesia, Jakarta, DKI, 10430, IndonesiaDepartment of Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, IndonesiaDepartment of Oral Biology and Oral Science Research Center, Faculty of Dentistry Universitas Indonesia, Jakarta, DKI, 10430, IndonesiaPorphyromonas gingivalis has virulence factors such as gingipain and lipopolysaccharide, causing bacteremia to reach the brain and activate neuroinflammatory release cytokines. This study analyzed the effect of the co-culture of neuron cells with P. gingivalis coated with anti-P. gingivalis antibodies against cytokines produced by neuron cells. The gene expressions of the TNF, IL1B, iNOS2 in neurons was evaluated using RT-qPCR. The results showed that P. gingivalis coated with anti-P. gingivalis antibody before co-culture with neuron cells could decrease the gene expression of TNF, IL1B, and iNOS2 of neuron cells.https://f1000research.com/articles/9-1499/v4
collection DOAJ
language English
format Article
sources DOAJ
author Endang Winiati Bachtiar
Citra F. Putri
Retno D. Soejoedono
Boy M. Bachtiar
spellingShingle Endang Winiati Bachtiar
Citra F. Putri
Retno D. Soejoedono
Boy M. Bachtiar
Expression of TNF, IL1B, and iNOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti-Porphyromonas gingivalis [version 4; peer review: 2 approved]
F1000Research
author_facet Endang Winiati Bachtiar
Citra F. Putri
Retno D. Soejoedono
Boy M. Bachtiar
author_sort Endang Winiati Bachtiar
title Expression of TNF, IL1B, and iNOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti-Porphyromonas gingivalis [version 4; peer review: 2 approved]
title_short Expression of TNF, IL1B, and iNOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti-Porphyromonas gingivalis [version 4; peer review: 2 approved]
title_full Expression of TNF, IL1B, and iNOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti-Porphyromonas gingivalis [version 4; peer review: 2 approved]
title_fullStr Expression of TNF, IL1B, and iNOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti-Porphyromonas gingivalis [version 4; peer review: 2 approved]
title_full_unstemmed Expression of TNF, IL1B, and iNOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti-Porphyromonas gingivalis [version 4; peer review: 2 approved]
title_sort expression of tnf, il1b, and inos2 in the neural cell after induced by porphyromonas gingivalis with and without coating antibody anti-porphyromonas gingivalis [version 4; peer review: 2 approved]
publisher F1000 Research Ltd
series F1000Research
issn 2046-1402
publishDate 2021-06-01
description Porphyromonas gingivalis has virulence factors such as gingipain and lipopolysaccharide, causing bacteremia to reach the brain and activate neuroinflammatory release cytokines. This study analyzed the effect of the co-culture of neuron cells with P. gingivalis coated with anti-P. gingivalis antibodies against cytokines produced by neuron cells. The gene expressions of the TNF, IL1B, iNOS2 in neurons was evaluated using RT-qPCR. The results showed that P. gingivalis coated with anti-P. gingivalis antibody before co-culture with neuron cells could decrease the gene expression of TNF, IL1B, and iNOS2 of neuron cells.
url https://f1000research.com/articles/9-1499/v4
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AT citrafputri expressionoftnfil1bandinos2intheneuralcellafterinducedbyporphyromonasgingivaliswithandwithoutcoatingantibodyantiporphyromonasgingivalisversion4peerreview2approved
AT retnodsoejoedono expressionoftnfil1bandinos2intheneuralcellafterinducedbyporphyromonasgingivaliswithandwithoutcoatingantibodyantiporphyromonasgingivalisversion4peerreview2approved
AT boymbachtiar expressionoftnfil1bandinos2intheneuralcellafterinducedbyporphyromonasgingivaliswithandwithoutcoatingantibodyantiporphyromonasgingivalisversion4peerreview2approved
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