Prevalence of <it>Listeria </it>species in camel sausages from retail markets in Aydin province in Turkey and RAPD analysis of <it>Listeria monocytogenes </it>Isolates

• Main Authors: Ozbey Gokben, Ertas Hasan, Kok Filiz
• Format: Article
• Language: English
• Published: BMC 2006-06-01
• Series: Irish Veterinary Journal
• Subjects: Camel sausage; <it>Listeria monocytogenes</it>; PCR; RAPD
• Online Access: http://www.irishvetjournal.org/content/59/6/342

<p>Abstract</p> <p>Samples were taken from 100 camel sausages from the different retail markets in Aydin province in the south-west of Turkey and they were tested for the presence of <it>Listeria </it>spp by biochemical methods. Samples were enriched using <it>Listeria </it>Enrichment Broth and they were inoculated onto <it>Listeria </it>Selective Agar. <it>Listeria monocytogenes </it>was isolated from nine samples (9%), <it>Listeria innocua </it>from 14 samples (14%) and <it>Listeria welshimeri </it>from two samples(2%). A 701 bp fragment of listeriolysin O sequence for <it>L. monocytogenes </it>was amplified using specific primers by polymerase chain reaction (PCR) for confirmation of the identification. A random primer (OPA-11) was used in a random amplified polymorphic DNA (RAPD) assay. This detected five different band profiles amongst the <it>L. monocytogenes </it>isolates, indicating a relatively large amount of genetic heterogeneity amongst the nine isolates. The study has highlighted the need for improved strategies for food safety, in particular appropriate hygienic precautions to avoid contamination of sausage during the manufacturing process and appropriate preservation techniques during storage and transport, to prevent transmission of <it>Listeria </it>spp to consumers at home and abroad.</p>