A New Approach for Characterizing the Intermediate Feature of α-Chymotrypsin Refolding by Hydrophobic Interaction Chromatography

A New Approach for Characterizing the Intermediate Feature of α-Chymotrypsin Refolding by Hydrophobic Interaction Chromatography

A new approach for characterizing the intermediate of urea-denatured α-chymotrypsin (α-Chy) by hydrophobic interaction chromatography (HIC) is presented. The contact surface region (Z, S), affinity (logI), and the character of interaction force (j) of the α-Chy to the stationary phase of HIC (STHIC)...

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Bibliographic Details
Main Authors: Zhenling Liu, Jianjun Li, Congyu Ke, Xindu Geng
Format: Article
Language:English
Published: MDPI AG 2009-02-01
Series:International Journal of Molecular Sciences
Subjects:
Protein folding
protein drugs
misfolding
intermediates
protein folding liquid chromatography
characterization
hydrophobic interaction chromatography
α-Chymotrypsin
stoichiometric displacement theory
Online Access:http://www.mdpi.com/1422-0067/10/2/616/

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http://www.mdpi.com/1422-0067/10/2/616/

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