Ultrastructural and Immunohistochemical Features of Telocytes in Placental Villi in Preeclampsia

• Main Authors: Natalia V. Nizyaeva, Tatiana V. Sukhacheva, Roman A. Serov, Galina V. Kulikova, Marina N. Nagovitsyna, Natalia E. Kan, Victor L. Tyutyunnik, Stanislav V. Pavlovich, Rimma A. Poltavtseva, Ekaterina L. Yarotskaya, Aleksandr I. Shchegolev, Gennadiy T. Sukhikh
• Format: Article
• Language: English
• Published: Nature Publishing Group 2018-02-01
• Series: Scientific Reports
• Online Access: https://doi.org/10.1038/s41598-018-21492-w

Abstract A new cell type, interstitial Cajal-like cell (ICLC), was recently described in different organs. The name was recently changed to telocytes (TCs), and their typical thin, long processes have been named telopodes (Tp). TCs regulate the contractile activity of smooth muscle cells and play a role in regulating vessel contractions. Although the placenta is not an innervated organ, we believe that TCs are present in the placenta. We studied placenta samples from physiological pregnancies and in different variants of preeclampsia (PE). We examined these samples using light microscopy of semi-thin sections, transmission electron microscopy, and immunohistochemistry. Immunohistochemical examination was performed with primary antibodies to CD34, CD117, SMA, and vimentin, and TMEM16a (DOG-1), the latter was used for the diagnosis of gastrointestinal stromal tumours (GIST) consisting of TCs. We have identified a heterogenetic population of ТСs in term placentas, as these cell types differed in their localization, immunophenotype and ultrastructural characteristics. We assume TMEM16a could be used as the marker for identification of TCs. In PE we have revealed telocyte-like cells with ultrastructural signs of fibrocytes (significant process thickening and the granular endoplasmic reticulum content was increased) and a loss of TMEM16a immunohistochemical staining.