Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae)
The composition of heterochromatin classes along the chromosomes of specimens from two populations of the fish Astyanax scabripinnis was examined using fluorescence banding with GC- and AT-DNA specific fluorochromes and fluorescence in situ hybridization (FISH) with an AT-rich satellite DNA (As51) p...
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Sociedade Brasileira de Genética
2004-01-01
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doaj-1f7f3448b8464aea96fd975e5d7ae95a2020-11-25T02:19:46ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852004-01-0127453654210.1590/S1415-47572004000400012Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae)Monique MantovaniLuciano Douglas dos Santos AbelCarlos Alberto MestrinerOrlando Moreira-FilhoThe composition of heterochromatin classes along the chromosomes of specimens from two populations of the fish Astyanax scabripinnis was examined using fluorescence banding with GC- and AT-DNA specific fluorochromes and fluorescence in situ hybridization (FISH) with an AT-rich satellite DNA (As51) probe. For the pericentromeric heterochromatin blocks neither GC/AT-DNA specific fluorochromes nor the FISH technique produce any response with chromosomes from either of the populations. On the other hand, the telomeric distal heterochromatin blocks of both populations fluoresced when the FISH technique was applied but showed distinct responses after GC-specific fluorochrome treatments, leading us to propose different structural arrangements of the FISH-positive heterochromatins. Such differences in chromosome banding patterns together with other karyotypic differences suggest differentiation of these populations at taxonomic level.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400012satellite DNAchromosome structurebase-specific fluorochromesheterochromatin heterogeneityfluorescence in situ hybridization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Monique Mantovani Luciano Douglas dos Santos Abel Carlos Alberto Mestriner Orlando Moreira-Filho |
spellingShingle |
Monique Mantovani Luciano Douglas dos Santos Abel Carlos Alberto Mestriner Orlando Moreira-Filho Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae) Genetics and Molecular Biology satellite DNA chromosome structure base-specific fluorochromes heterochromatin heterogeneity fluorescence in situ hybridization |
author_facet |
Monique Mantovani Luciano Douglas dos Santos Abel Carlos Alberto Mestriner Orlando Moreira-Filho |
author_sort |
Monique Mantovani |
title |
Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae) |
title_short |
Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae) |
title_full |
Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae) |
title_fullStr |
Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae) |
title_full_unstemmed |
Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae) |
title_sort |
evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of astyanax scabripinnis (pisces, characidae) |
publisher |
Sociedade Brasileira de Genética |
series |
Genetics and Molecular Biology |
issn |
1415-4757 1678-4685 |
publishDate |
2004-01-01 |
description |
The composition of heterochromatin classes along the chromosomes of specimens from two populations of the fish Astyanax scabripinnis was examined using fluorescence banding with GC- and AT-DNA specific fluorochromes and fluorescence in situ hybridization (FISH) with an AT-rich satellite DNA (As51) probe. For the pericentromeric heterochromatin blocks neither GC/AT-DNA specific fluorochromes nor the FISH technique produce any response with chromosomes from either of the populations. On the other hand, the telomeric distal heterochromatin blocks of both populations fluoresced when the FISH technique was applied but showed distinct responses after GC-specific fluorochrome treatments, leading us to propose different structural arrangements of the FISH-positive heterochromatins. Such differences in chromosome banding patterns together with other karyotypic differences suggest differentiation of these populations at taxonomic level. |
topic |
satellite DNA chromosome structure base-specific fluorochromes heterochromatin heterogeneity fluorescence in situ hybridization |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400012 |
work_keys_str_mv |
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