Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae)

The composition of heterochromatin classes along the chromosomes of specimens from two populations of the fish Astyanax scabripinnis was examined using fluorescence banding with GC- and AT-DNA specific fluorochromes and fluorescence in situ hybridization (FISH) with an AT-rich satellite DNA (As51) p...

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Main Authors: Monique Mantovani, Luciano Douglas dos Santos Abel, Carlos Alberto Mestriner, Orlando Moreira-Filho
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2004-01-01
Series:Genetics and Molecular Biology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400012
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spelling doaj-1f7f3448b8464aea96fd975e5d7ae95a2020-11-25T02:19:46ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852004-01-0127453654210.1590/S1415-47572004000400012Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae)Monique MantovaniLuciano Douglas dos Santos AbelCarlos Alberto MestrinerOrlando Moreira-FilhoThe composition of heterochromatin classes along the chromosomes of specimens from two populations of the fish Astyanax scabripinnis was examined using fluorescence banding with GC- and AT-DNA specific fluorochromes and fluorescence in situ hybridization (FISH) with an AT-rich satellite DNA (As51) probe. For the pericentromeric heterochromatin blocks neither GC/AT-DNA specific fluorochromes nor the FISH technique produce any response with chromosomes from either of the populations. On the other hand, the telomeric distal heterochromatin blocks of both populations fluoresced when the FISH technique was applied but showed distinct responses after GC-specific fluorochrome treatments, leading us to propose different structural arrangements of the FISH-positive heterochromatins. Such differences in chromosome banding patterns together with other karyotypic differences suggest differentiation of these populations at taxonomic level.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400012satellite DNAchromosome structurebase-specific fluorochromesheterochromatin heterogeneityfluorescence in situ hybridization
collection DOAJ
language English
format Article
sources DOAJ
author Monique Mantovani
Luciano Douglas dos Santos Abel
Carlos Alberto Mestriner
Orlando Moreira-Filho
spellingShingle Monique Mantovani
Luciano Douglas dos Santos Abel
Carlos Alberto Mestriner
Orlando Moreira-Filho
Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae)
Genetics and Molecular Biology
satellite DNA
chromosome structure
base-specific fluorochromes
heterochromatin heterogeneity
fluorescence in situ hybridization
author_facet Monique Mantovani
Luciano Douglas dos Santos Abel
Carlos Alberto Mestriner
Orlando Moreira-Filho
author_sort Monique Mantovani
title Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae)
title_short Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae)
title_full Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae)
title_fullStr Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae)
title_full_unstemmed Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae)
title_sort evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of astyanax scabripinnis (pisces, characidae)
publisher Sociedade Brasileira de Genética
series Genetics and Molecular Biology
issn 1415-4757
1678-4685
publishDate 2004-01-01
description The composition of heterochromatin classes along the chromosomes of specimens from two populations of the fish Astyanax scabripinnis was examined using fluorescence banding with GC- and AT-DNA specific fluorochromes and fluorescence in situ hybridization (FISH) with an AT-rich satellite DNA (As51) probe. For the pericentromeric heterochromatin blocks neither GC/AT-DNA specific fluorochromes nor the FISH technique produce any response with chromosomes from either of the populations. On the other hand, the telomeric distal heterochromatin blocks of both populations fluoresced when the FISH technique was applied but showed distinct responses after GC-specific fluorochrome treatments, leading us to propose different structural arrangements of the FISH-positive heterochromatins. Such differences in chromosome banding patterns together with other karyotypic differences suggest differentiation of these populations at taxonomic level.
topic satellite DNA
chromosome structure
base-specific fluorochromes
heterochromatin heterogeneity
fluorescence in situ hybridization
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400012
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