Solving the mysteries of the bacterial cell – application of novel techniques in fluorescence microscopy
We have reviewed how the development of fluorescent markers, triggered by the discovery of green fluorescence protein and its other color variants leading to the establishment of methods for studies of protein interactions with application of fluorescent proteins, affected the view of bacterial cell...
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Index Copernicus International S.A.
2011-01-01
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| Series: | Postępy Higieny i Medycyny Doświadczalnej |
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| Online Access: | http://journals.indexcopernicus.com/fulltxt.php?ICID=933713 |
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doaj-1f6c97defc78484695142798593fab2f2020-11-24T23:32:26ZengIndex Copernicus International S.A.Postępy Higieny i Medycyny Doświadczalnej0032-54491732-26932011-01-0165114123Solving the mysteries of the bacterial cell – application of novel techniques in fluorescence microscopyMagdalena DonczewKatarzyna GindaJolanta Zakrzewska-CzerwińskaDagmara JakimowiczWe have reviewed how the development of fluorescent markers, triggered by the discovery of green fluorescence protein and its other color variants leading to the establishment of methods for studies of protein interactions with application of fluorescent proteins, affected the view of bacterial cell organization. Application of the new microscopic methods allowed localization of proteins and chromosomal regions, and observation of their migration in real time. These studies revealed the spatial organization of bacterial cells which includes specific subcellular localization of proteins, the presence of dynamic cytoskeletal structures, orchestrated and active segregation of chromosomes, and spatiotemporal gene regulation.http://journals.indexcopernicus.com/fulltxt.php?ICID=933713fluorescence microscopyfluorescent proteinsFRETFRAPbacterial cytoskeletonorganization of bacterial cell |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Magdalena Donczew Katarzyna Ginda Jolanta Zakrzewska-Czerwińska Dagmara Jakimowicz |
| spellingShingle |
Magdalena Donczew Katarzyna Ginda Jolanta Zakrzewska-Czerwińska Dagmara Jakimowicz Solving the mysteries of the bacterial cell – application of novel techniques in fluorescence microscopy Postępy Higieny i Medycyny Doświadczalnej fluorescence microscopy fluorescent proteins FRET FRAP bacterial cytoskeleton organization of bacterial cell |
| author_facet |
Magdalena Donczew Katarzyna Ginda Jolanta Zakrzewska-Czerwińska Dagmara Jakimowicz |
| author_sort |
Magdalena Donczew |
| title |
Solving the mysteries of the bacterial cell – application of novel techniques in fluorescence microscopy |
| title_short |
Solving the mysteries of the bacterial cell – application of novel techniques in fluorescence microscopy |
| title_full |
Solving the mysteries of the bacterial cell – application of novel techniques in fluorescence microscopy |
| title_fullStr |
Solving the mysteries of the bacterial cell – application of novel techniques in fluorescence microscopy |
| title_full_unstemmed |
Solving the mysteries of the bacterial cell – application of novel techniques in fluorescence microscopy |
| title_sort |
solving the mysteries of the bacterial cell – application of novel techniques in fluorescence microscopy |
| publisher |
Index Copernicus International S.A. |
| series |
Postępy Higieny i Medycyny Doświadczalnej |
| issn |
0032-5449 1732-2693 |
| publishDate |
2011-01-01 |
| description |
We have reviewed how the development of fluorescent markers, triggered by the discovery of green fluorescence protein and its other color variants leading to the establishment of methods for studies of protein interactions with application of fluorescent proteins, affected the view of bacterial cell organization. Application of the new microscopic methods allowed localization of proteins and chromosomal regions, and observation of their migration in real time. These studies revealed the spatial organization of bacterial cells which includes specific subcellular localization of proteins, the presence of dynamic cytoskeletal structures, orchestrated and active segregation of chromosomes, and spatiotemporal gene regulation. |
| topic |
fluorescence microscopy fluorescent proteins FRET FRAP bacterial cytoskeleton organization of bacterial cell |
| url |
http://journals.indexcopernicus.com/fulltxt.php?ICID=933713 |
| work_keys_str_mv |
AT magdalenadonczew solvingthemysteriesofthebacterialcellapplicationofnoveltechniquesinfluorescencemicroscopy AT katarzynaginda solvingthemysteriesofthebacterialcellapplicationofnoveltechniquesinfluorescencemicroscopy AT jolantazakrzewskaczerwinska solvingthemysteriesofthebacterialcellapplicationofnoveltechniquesinfluorescencemicroscopy AT dagmarajakimowicz solvingthemysteriesofthebacterialcellapplicationofnoveltechniquesinfluorescencemicroscopy |
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1725534214181355520 |