M-cholinoreactivity dynamics in frog myocardium exposed to serum from human umbilical blood

• Main Authors: V. I. Zirkin, A. N. Trukhin, E. N. Sizova, S. A. Dvorjanskiy
• Format: Article
• Language: Russian
• Published: «FIRMA «SILICEA» LLC 2004-04-01
• Series: Российский кардиологический журнал
• Subjects: frog miocardium; acetylcholine; serum from umbilical blood; m3cholinoreativity dynamics
• Online Access: https://russjcardiol.elpub.ru/jour/article/view/1961

In experiments with isolated hearts from 31 frogs (R. ridibunda), acetylcholine (10-7, 10-6, 10-5g/ml) in a dose-dependent manner reduced contraction amplitude during electrostimulation (30 s, 1 Gz, 5 ms, 5-10 W). The time to achieve maximal amplitude did not change, that meant the serum had negative inotropic effect, weakening chrono-inotropic correlations. Serum from umbilical blood in titer 1 : 100 (SUB-1: 100) did not affect contraction amplitude and chrono-inotropic correlations. But, due to endogenous blocker of M-cholinoreceptors (EMCRB), it decreased myocardial M-cholinoreactivity, demonstrating M-cholinoblocking properties. In particular, the serum shifted the dose-effect curve rightand downward, increasing acetylcholine Kd by more than 3 times. Serum acetylcholine affinity to M-CHR diminished, and M-cholinergic influence on the heart became less effective. The serum decreased acetylcholine potential (10-7 and 10-6 g/ml) to weaken chrono-inotropic correlations. In some cases, SUB-1: 100, due to endogenous M-cholinorecepor stabilizer (EMCRS), increased negative inotropic effect of acetylcholine. The nature of EMCRB and EMCRS, together with their possible normal and pathologic role in regulating the heart function, is discussed.