M-cholinoreactivity dynamics in frog myocardium exposed to serum from human umbilical blood
In experiments with isolated hearts from 31 frogs (R. ridibunda), acetylcholine (10-7, 10-6, 10-5g/ml) in a dose-dependent manner reduced contraction amplitude during electrostimulation (30 s, 1 Gz, 5 ms, 5-10 W). The time to achieve maximal amplitude did not change, that meant the serum had negativ...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Russian |
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«FIRMA «SILICEA» LLC
2004-04-01
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| Series: | Российский кардиологический журнал |
| Subjects: | |
| Online Access: | https://russjcardiol.elpub.ru/jour/article/view/1961 |
| Summary: | In experiments with isolated hearts from 31 frogs (R. ridibunda), acetylcholine (10-7, 10-6, 10-5g/ml) in a dose-dependent manner reduced contraction amplitude during electrostimulation (30 s, 1 Gz, 5 ms, 5-10 W). The time to achieve maximal amplitude did not change, that meant the serum had negative inotropic effect, weakening chrono-inotropic correlations. Serum from umbilical blood in titer 1 : 100 (SUB-1: 100) did not affect contraction amplitude and chrono-inotropic correlations. But, due to endogenous blocker of M-cholinoreceptors (EMCRB), it decreased myocardial M-cholinoreactivity, demonstrating M-cholinoblocking properties. In particular, the serum shifted the dose-effect curve rightand downward, increasing acetylcholine Kd by more than 3 times. Serum acetylcholine affinity to M-CHR diminished, and M-cholinergic influence on the heart became less effective. The serum decreased acetylcholine potential (10-7 and 10-6 g/ml) to weaken chrono-inotropic correlations. In some cases, SUB-1: 100, due to endogenous M-cholinorecepor stabilizer (EMCRS), increased negative inotropic effect of acetylcholine. The nature of EMCRB and EMCRS, together with their possible normal and pathologic role in regulating the heart function, is discussed. |
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| ISSN: | 1560-4071 2618-7620 |