THE REFERENCE VALUES OF THE MAIN BIOCHEMICAL PARAMETERS OF THE HEMOLYMPH OF APIS MELLIFERA CARPATHICA IN SOUTH-EASTERN ROMANIA

• Main Authors: AGRIPINA SAPCALIU, ELIZA CAUIA, ION GURESOAIE, DAN CONDUR, ION RADOI
• Format: Article
• Language: English
• Published: Agroprint Timisoara 2007-10-01
• Series: Scientific Papers Animal Science and Biotechnologies
• Subjects: apis mellifera carpathica honeybees; hemolymph; biochemical parameters
• Online Access: http://spasb.ro/index.php/spasb/article/view/1368
• ISSN: 1841-9364; 2344-4576

Although biochemical analyses of the blood were and are still used for the routinediagnosis and especially for the metabolic survey in farm animals, such analyses, maybe applied for the honeybee hemolymph (as a paraclinic examination). The aim of thisexperimental study was to investigate and to determine the reference values of themain biochemical parameters in the hemolymph of the healthy honeybees of Apismellifera carpathica. The honeybee samples were collected in order to analyze thehealth condition of the respective colony. All the samples coming from sick colonieswere removed, only healthy adult honeybees coming from strong colonies were keptand used for hemolymph collection. By special methods, samples of hemolymph (300μl/sample) collected from about 50 individuals, were analyzed both during the activeseason (spring/summer) and the inactive season (autumn/winter). The study wascarried out on 50 samples of undiluted hemolymph taken from a total number of about2,500 honeybees. The following 21 biochemical parameters were analyzed: GLU(mg/dl), HDL-c (mg/dl), ALP (UI/l), T-cho (mg/dl), Tprot (mg/dl), Alb. (g/dl), BUN(mg/dl), LDH (UI/l), CPK (UI/l), Mg (mg/dl), FRA (μm/l), IP (mg/dl), GGT (UI/l),GOT (UI/l), GPT (UI/l), Ca (mg/dl), Cre (mg/dl), Amy (UI/l), T–BIL (mg/dl), TG(mg/dl), UA (mg/dl). The test was carried out after the collection and processing of thesamples using the SPOTCHEM EZSP4430, equipment with dry kits, the slides technique,respectively.