Identification of BmBrat gene in silkworm, Bombyx mori
NHL protein family is evolutionarily conserved in either vertebrates or invertebrates. It has been extensively studied in mammals for the function of regulating cell proliferation and differentiation. Brat, known as the homologue of TRIM in insects, may have the same function. In this paper, we ide...
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University of Modena and Reggio Emilia
2018-04-01
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| Series: | Invertebrate Survival Journal |
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| Online Access: | https://isj02.unimore.it/index.php/ISJ/article/view/420 |
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doaj-1f18e95cae594fe5843d0bc75c9074d12020-12-02T18:27:00ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2018-04-01151Identification of BmBrat gene in silkworm, Bombyx moriX X Ke0H Gao1K Chen2L Zhang3S Chen4X Wang5Z Wei6L Yang7H Cui8State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. ChinaDepartment 6 of Research Institute of Surgery, State Key Laboratory of Trauma, Burns and Combined Injury, Daping Hospital, Army Medical University, Chongqing 400042, P.R. ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. China NHL protein family is evolutionarily conserved in either vertebrates or invertebrates. It has been extensively studied in mammals for the function of regulating cell proliferation and differentiation. Brat, known as the homologue of TRIM in insects, may have the same function. In this paper, we identified and characterized BmBrat in silkworm. The gene expression profile indicated that BmBrat was expressed in all tissues and the expression was especially high in ovary and head at larval stage. The expression profile was also detected at different stages of embryo development, and reached a peak at 4th and 5th days of the embryonic period. Subcellular localization of BmBrat in hemocytes revealed that it was specifically expressed in cytoplasm. Over-expression of BmBrat in silkworm significantly reduced the rate of cell proliferation by BrdU staining. This finding suggested that BmBrat played an important role in cell processes. https://isj02.unimore.it/index.php/ISJ/article/view/420silkwormBmBratgene expression profilesubcellular localization |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
X X Ke H Gao K Chen L Zhang S Chen X Wang Z Wei L Yang H Cui |
| spellingShingle |
X X Ke H Gao K Chen L Zhang S Chen X Wang Z Wei L Yang H Cui Identification of BmBrat gene in silkworm, Bombyx mori Invertebrate Survival Journal silkworm BmBrat gene expression profile subcellular localization |
| author_facet |
X X Ke H Gao K Chen L Zhang S Chen X Wang Z Wei L Yang H Cui |
| author_sort |
X X Ke |
| title |
Identification of BmBrat gene in silkworm, Bombyx mori |
| title_short |
Identification of BmBrat gene in silkworm, Bombyx mori |
| title_full |
Identification of BmBrat gene in silkworm, Bombyx mori |
| title_fullStr |
Identification of BmBrat gene in silkworm, Bombyx mori |
| title_full_unstemmed |
Identification of BmBrat gene in silkworm, Bombyx mori |
| title_sort |
identification of bmbrat gene in silkworm, bombyx mori |
| publisher |
University of Modena and Reggio Emilia |
| series |
Invertebrate Survival Journal |
| issn |
1824-307X |
| publishDate |
2018-04-01 |
| description |
NHL protein family is evolutionarily conserved in either vertebrates or invertebrates. It has been extensively studied in mammals for the function of regulating cell proliferation and differentiation. Brat, known as the homologue of TRIM in insects, may have the same function. In this paper, we identified and characterized BmBrat in silkworm. The gene expression profile indicated that BmBrat was expressed in all tissues and the expression was especially high in ovary and head at larval stage. The expression profile was also detected at different stages of embryo development, and reached a peak at 4th and 5th days of the embryonic period. Subcellular localization of BmBrat in hemocytes revealed that it was specifically expressed in cytoplasm. Over-expression of BmBrat in silkworm significantly reduced the rate of cell proliferation by BrdU staining. This finding suggested that BmBrat played an important role in cell processes.
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| topic |
silkworm BmBrat gene expression profile subcellular localization |
| url |
https://isj02.unimore.it/index.php/ISJ/article/view/420 |
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