A Novel WRKY Transcription Factor HmoWRKY40 Associated with Betalain Biosynthesis in Pitaya (<i>Hylocereus monacanthus</i>) through Regulating <i>HmoCYP76AD1</i>
Betalains are water-soluble nitrogen-containing pigments with multiple bioactivities. Pitaya is the only large-scale commercially grown fruit containing abundant betalains for consumers. However, the upstream regulators in betalain biosynthesis are still not clear. In this study, HmoWRKY40, a novel...
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MDPI AG
2021-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/4/2171 |
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doaj-1f092eb3212e44fbba035ac923888e61 |
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Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Lulu Zhang Canbin Chen Fangfang Xie Qingzhu Hua Zhike Zhang Rong Zhang Jianye Chen Jietang Zhao Guibing Hu Yonghua Qin |
| spellingShingle |
Lulu Zhang Canbin Chen Fangfang Xie Qingzhu Hua Zhike Zhang Rong Zhang Jianye Chen Jietang Zhao Guibing Hu Yonghua Qin A Novel WRKY Transcription Factor HmoWRKY40 Associated with Betalain Biosynthesis in Pitaya (<i>Hylocereus monacanthus</i>) through Regulating <i>HmoCYP76AD1</i> International Journal of Molecular Sciences Pitaya betalain biosynthesis transcription factor HmoWRKY40 VIGS |
| author_facet |
Lulu Zhang Canbin Chen Fangfang Xie Qingzhu Hua Zhike Zhang Rong Zhang Jianye Chen Jietang Zhao Guibing Hu Yonghua Qin |
| author_sort |
Lulu Zhang |
| title |
A Novel WRKY Transcription Factor HmoWRKY40 Associated with Betalain Biosynthesis in Pitaya (<i>Hylocereus monacanthus</i>) through Regulating <i>HmoCYP76AD1</i> |
| title_short |
A Novel WRKY Transcription Factor HmoWRKY40 Associated with Betalain Biosynthesis in Pitaya (<i>Hylocereus monacanthus</i>) through Regulating <i>HmoCYP76AD1</i> |
| title_full |
A Novel WRKY Transcription Factor HmoWRKY40 Associated with Betalain Biosynthesis in Pitaya (<i>Hylocereus monacanthus</i>) through Regulating <i>HmoCYP76AD1</i> |
| title_fullStr |
A Novel WRKY Transcription Factor HmoWRKY40 Associated with Betalain Biosynthesis in Pitaya (<i>Hylocereus monacanthus</i>) through Regulating <i>HmoCYP76AD1</i> |
| title_full_unstemmed |
A Novel WRKY Transcription Factor HmoWRKY40 Associated with Betalain Biosynthesis in Pitaya (<i>Hylocereus monacanthus</i>) through Regulating <i>HmoCYP76AD1</i> |
| title_sort |
novel wrky transcription factor hmowrky40 associated with betalain biosynthesis in pitaya (<i>hylocereus monacanthus</i>) through regulating <i>hmocyp76ad1</i> |
| publisher |
MDPI AG |
| series |
International Journal of Molecular Sciences |
| issn |
1661-6596 1422-0067 |
| publishDate |
2021-02-01 |
| description |
Betalains are water-soluble nitrogen-containing pigments with multiple bioactivities. Pitaya is the only large-scale commercially grown fruit containing abundant betalains for consumers. However, the upstream regulators in betalain biosynthesis are still not clear. In this study, HmoWRKY40, a novel WRKY transcription factor, was obtained from the transcriptome data of pitaya (<i>Hylocereus monacanthus</i>). HmoWRKY40 is a member of the Group IIa WRKY family, containing a conserved WRKY motif, and it is located in the nucleus. The betalain contents and expression levels of HmoWRKY40 increased rapidly during the coloration of pitaya and reached their maximums on the 23rd day after artificial pollination (DAAP). Yeast one-hybrid and transient expression assays showed that HmoWRKY40 could bind and activate the promoter of <i>HmoCYP76AD1</i>. Silencing the <i>HmoWRKY40</i> gene resulted in a significant reduction of betacyanin contents. These results indicate that HmoWRKY40 transcriptionally activates <i>HmoCYP76AD,</i> which is involved in the regulation of pitaya betalain biosynthesis. The results of the present study provide new regulatory networks related to betalain biosynthesis in pitaya. |
| topic |
Pitaya betalain biosynthesis transcription factor HmoWRKY40 VIGS |
| url |
https://www.mdpi.com/1422-0067/22/4/2171 |
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doaj-1f092eb3212e44fbba035ac923888e612021-02-23T00:03:06ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-02-01222171217110.3390/ijms22042171A Novel WRKY Transcription Factor HmoWRKY40 Associated with Betalain Biosynthesis in Pitaya (<i>Hylocereus monacanthus</i>) through Regulating <i>HmoCYP76AD1</i>Lulu Zhang0Canbin Chen1Fangfang Xie2Qingzhu Hua3Zhike Zhang4Rong Zhang5Jianye Chen6Jietang Zhao7Guibing Hu8Yonghua Qin9State Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaBetalains are water-soluble nitrogen-containing pigments with multiple bioactivities. Pitaya is the only large-scale commercially grown fruit containing abundant betalains for consumers. However, the upstream regulators in betalain biosynthesis are still not clear. In this study, HmoWRKY40, a novel WRKY transcription factor, was obtained from the transcriptome data of pitaya (<i>Hylocereus monacanthus</i>). HmoWRKY40 is a member of the Group IIa WRKY family, containing a conserved WRKY motif, and it is located in the nucleus. The betalain contents and expression levels of HmoWRKY40 increased rapidly during the coloration of pitaya and reached their maximums on the 23rd day after artificial pollination (DAAP). Yeast one-hybrid and transient expression assays showed that HmoWRKY40 could bind and activate the promoter of <i>HmoCYP76AD1</i>. Silencing the <i>HmoWRKY40</i> gene resulted in a significant reduction of betacyanin contents. These results indicate that HmoWRKY40 transcriptionally activates <i>HmoCYP76AD,</i> which is involved in the regulation of pitaya betalain biosynthesis. The results of the present study provide new regulatory networks related to betalain biosynthesis in pitaya.https://www.mdpi.com/1422-0067/22/4/2171Pitayabetalain biosynthesistranscription factorHmoWRKY40VIGS |