Transgressive segregations for agronomic improvement using interspecific crosses between C. arietinum L. x C. reticulatum Ladiz. and C. arietinum L. x C. echinospermum Davis species.

• Main Authors: Mohar Singh, Savita Rani, Nikhil Malhotra, Gopal Katna, Ashutosh Sarker
• Format: Article
• Language: English
• Published: Public Library of Science (PLoS) 2018-01-01
• Series: PLoS ONE
• Online Access: http://europepmc.org/articles/PMC6128482?pdf=render

The wild species of chickpea have tremendous potential for enhancing genetic gains of cultigen and have resistant accessions against major biotic and abiotic stresses. In the present study, two wild annual accessions, one each of C. reticulatum Ladiz. (ILWC 229) and C. echinospermum Davis (ILWC 246) were assessed for their agro-morphological features and hybridized with different cultivated varieties (BGD 72, PBG 5, ICKG 96029, Pusa 372 and JG 11) of chickpea. Fertile F1 plants were developed as revealed by their normal meiotic chromosomal configuration including high pollen stainability percentage and seed set. The effect of genetic and non-genetic factors on crossability performance with respect to pod and seed set was also evident under two growing conditions of North-Western Indian Himalayas. The segregation analysis using F2 phenotypic ratio of some distinct morphological (plant growth habit, stem pigmentation at seedling stage and testa texture) characters indicated their monogenic inheritance pattern. The study would also be useful to chickpea breeders to identify true to type interspecific plants. Further, the F1, F2 and F3 generations of all seven crosses along with parents were evaluated under natural field condition to determine the extent of variability created into the cultivated background of chickpea. There was a wide range of variation in F3 population against cold stress, suggesting selection of tolerant recombinant lines at an early stage. We also studied fruitful heterosis (%) as a useful approach, instead of residual heterosis to identify better performing transgressive segregants. The values of most of the interspecific crosses for important traits assessed in F2 and F3 generations were higher than that of better parent, suggesting isolation of inbred vigour for pod numbers and earliness. The results indicated that wild Cicer annual accessions of C. reticulatum and C. echinospermum species can be exploited after proper screening for traits of interest for diversification of cultivated gene pool and subsequent use in chickpea improvement.