Genetic structure in dwarf bamboo (Bashania fangiana) clonal populations with different genet ages.

Amplified fragment length polymorphism (AFLP) fingerprints were used to reveal genotypic diversity of dwarf bamboo (Bashania fangiana) clonal populations with two different genet ages (≤30 years versus >70 years) at Wolong National Natural Reserve, Sichuan province, China. We generated AFLP finge...

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Main Authors: Qing-qing Ma, Hui-xing Song, Shi-qiang Zhou, Wan-qin Yang, De-sheng Li, Jin-song Chen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3828345?pdf=render
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spelling doaj-1ee5ca57f0eb4fc5a608f0f1ed6a3b452020-11-25T00:02:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01811e7878410.1371/journal.pone.0078784Genetic structure in dwarf bamboo (Bashania fangiana) clonal populations with different genet ages.Qing-qing MaHui-xing SongShi-qiang ZhouWan-qin YangDe-sheng LiJin-song ChenAmplified fragment length polymorphism (AFLP) fingerprints were used to reveal genotypic diversity of dwarf bamboo (Bashania fangiana) clonal populations with two different genet ages (≤30 years versus >70 years) at Wolong National Natural Reserve, Sichuan province, China. We generated AFLP fingerprints for 96 leaf samples, collected at 30 m intervals in the two populations, using ten selective primer pairs. A total of 92 genotypes were identified from the both populations. The mean proportion of distinguishable genotypes (G/N) was 0.9583 (0.9375 to 0.9792) and Simpson's index of diversity (D) was 0.9982 (0.9973 to 0.9991). So, two B. fangiana populations were multiclonal and highly diverse. The largest single clone may occur over a distance of about 30 m. Our results demonstrated that the genotypic diversity and genet density of B. fangiana clonal population did not change significantly (47 versus 45) with genet aging and low partitioned genetic differentiation was between the two populations (Gst = 0.0571). The analysis of molecular variance consistently showed that a large proportion of the genetic variation (87.79%) existed among the individuals within populations, whereas only 12.21% were found among populations. In addition, the high level of genotypic diversity in the two populations implies that the further works were needed to investigate the reasons for the poor seed set in B. fangiana after flowering.http://europepmc.org/articles/PMC3828345?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Qing-qing Ma
Hui-xing Song
Shi-qiang Zhou
Wan-qin Yang
De-sheng Li
Jin-song Chen
spellingShingle Qing-qing Ma
Hui-xing Song
Shi-qiang Zhou
Wan-qin Yang
De-sheng Li
Jin-song Chen
Genetic structure in dwarf bamboo (Bashania fangiana) clonal populations with different genet ages.
PLoS ONE
author_facet Qing-qing Ma
Hui-xing Song
Shi-qiang Zhou
Wan-qin Yang
De-sheng Li
Jin-song Chen
author_sort Qing-qing Ma
title Genetic structure in dwarf bamboo (Bashania fangiana) clonal populations with different genet ages.
title_short Genetic structure in dwarf bamboo (Bashania fangiana) clonal populations with different genet ages.
title_full Genetic structure in dwarf bamboo (Bashania fangiana) clonal populations with different genet ages.
title_fullStr Genetic structure in dwarf bamboo (Bashania fangiana) clonal populations with different genet ages.
title_full_unstemmed Genetic structure in dwarf bamboo (Bashania fangiana) clonal populations with different genet ages.
title_sort genetic structure in dwarf bamboo (bashania fangiana) clonal populations with different genet ages.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2013-01-01
description Amplified fragment length polymorphism (AFLP) fingerprints were used to reveal genotypic diversity of dwarf bamboo (Bashania fangiana) clonal populations with two different genet ages (≤30 years versus >70 years) at Wolong National Natural Reserve, Sichuan province, China. We generated AFLP fingerprints for 96 leaf samples, collected at 30 m intervals in the two populations, using ten selective primer pairs. A total of 92 genotypes were identified from the both populations. The mean proportion of distinguishable genotypes (G/N) was 0.9583 (0.9375 to 0.9792) and Simpson's index of diversity (D) was 0.9982 (0.9973 to 0.9991). So, two B. fangiana populations were multiclonal and highly diverse. The largest single clone may occur over a distance of about 30 m. Our results demonstrated that the genotypic diversity and genet density of B. fangiana clonal population did not change significantly (47 versus 45) with genet aging and low partitioned genetic differentiation was between the two populations (Gst = 0.0571). The analysis of molecular variance consistently showed that a large proportion of the genetic variation (87.79%) existed among the individuals within populations, whereas only 12.21% were found among populations. In addition, the high level of genotypic diversity in the two populations implies that the further works were needed to investigate the reasons for the poor seed set in B. fangiana after flowering.
url http://europepmc.org/articles/PMC3828345?pdf=render
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AT wanqinyang geneticstructureindwarfbamboobashaniafangianaclonalpopulationswithdifferentgenetages
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