Genetic structure in dwarf bamboo (Bashania fangiana) clonal populations with different genet ages.
Amplified fragment length polymorphism (AFLP) fingerprints were used to reveal genotypic diversity of dwarf bamboo (Bashania fangiana) clonal populations with two different genet ages (≤30 years versus >70 years) at Wolong National Natural Reserve, Sichuan province, China. We generated AFLP finge...
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doaj-1ee5ca57f0eb4fc5a608f0f1ed6a3b452020-11-25T00:02:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01811e7878410.1371/journal.pone.0078784Genetic structure in dwarf bamboo (Bashania fangiana) clonal populations with different genet ages.Qing-qing MaHui-xing SongShi-qiang ZhouWan-qin YangDe-sheng LiJin-song ChenAmplified fragment length polymorphism (AFLP) fingerprints were used to reveal genotypic diversity of dwarf bamboo (Bashania fangiana) clonal populations with two different genet ages (≤30 years versus >70 years) at Wolong National Natural Reserve, Sichuan province, China. We generated AFLP fingerprints for 96 leaf samples, collected at 30 m intervals in the two populations, using ten selective primer pairs. A total of 92 genotypes were identified from the both populations. The mean proportion of distinguishable genotypes (G/N) was 0.9583 (0.9375 to 0.9792) and Simpson's index of diversity (D) was 0.9982 (0.9973 to 0.9991). So, two B. fangiana populations were multiclonal and highly diverse. The largest single clone may occur over a distance of about 30 m. Our results demonstrated that the genotypic diversity and genet density of B. fangiana clonal population did not change significantly (47 versus 45) with genet aging and low partitioned genetic differentiation was between the two populations (Gst = 0.0571). The analysis of molecular variance consistently showed that a large proportion of the genetic variation (87.79%) existed among the individuals within populations, whereas only 12.21% were found among populations. In addition, the high level of genotypic diversity in the two populations implies that the further works were needed to investigate the reasons for the poor seed set in B. fangiana after flowering.http://europepmc.org/articles/PMC3828345?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qing-qing Ma Hui-xing Song Shi-qiang Zhou Wan-qin Yang De-sheng Li Jin-song Chen |
spellingShingle |
Qing-qing Ma Hui-xing Song Shi-qiang Zhou Wan-qin Yang De-sheng Li Jin-song Chen Genetic structure in dwarf bamboo (Bashania fangiana) clonal populations with different genet ages. PLoS ONE |
author_facet |
Qing-qing Ma Hui-xing Song Shi-qiang Zhou Wan-qin Yang De-sheng Li Jin-song Chen |
author_sort |
Qing-qing Ma |
title |
Genetic structure in dwarf bamboo (Bashania fangiana) clonal populations with different genet ages. |
title_short |
Genetic structure in dwarf bamboo (Bashania fangiana) clonal populations with different genet ages. |
title_full |
Genetic structure in dwarf bamboo (Bashania fangiana) clonal populations with different genet ages. |
title_fullStr |
Genetic structure in dwarf bamboo (Bashania fangiana) clonal populations with different genet ages. |
title_full_unstemmed |
Genetic structure in dwarf bamboo (Bashania fangiana) clonal populations with different genet ages. |
title_sort |
genetic structure in dwarf bamboo (bashania fangiana) clonal populations with different genet ages. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
Amplified fragment length polymorphism (AFLP) fingerprints were used to reveal genotypic diversity of dwarf bamboo (Bashania fangiana) clonal populations with two different genet ages (≤30 years versus >70 years) at Wolong National Natural Reserve, Sichuan province, China. We generated AFLP fingerprints for 96 leaf samples, collected at 30 m intervals in the two populations, using ten selective primer pairs. A total of 92 genotypes were identified from the both populations. The mean proportion of distinguishable genotypes (G/N) was 0.9583 (0.9375 to 0.9792) and Simpson's index of diversity (D) was 0.9982 (0.9973 to 0.9991). So, two B. fangiana populations were multiclonal and highly diverse. The largest single clone may occur over a distance of about 30 m. Our results demonstrated that the genotypic diversity and genet density of B. fangiana clonal population did not change significantly (47 versus 45) with genet aging and low partitioned genetic differentiation was between the two populations (Gst = 0.0571). The analysis of molecular variance consistently showed that a large proportion of the genetic variation (87.79%) existed among the individuals within populations, whereas only 12.21% were found among populations. In addition, the high level of genotypic diversity in the two populations implies that the further works were needed to investigate the reasons for the poor seed set in B. fangiana after flowering. |
url |
http://europepmc.org/articles/PMC3828345?pdf=render |
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