| Summary: | The objective of this study was to determine the impact of <i>Ageratina adenophora (A. adenophora)</i> on splenic immune function in a rat model. Rats were fed with 10 g/100 g normal feed and an experimental feed, which was composed of 3:7<i> A. adenophora</i> powder and normal feed for 60 days. On days 14, 28, and 60, subsets of rats (<i>n</i> = 8 rats/group/time point) were selected for blood and spleen tissue sample collection. The results showed that the proportion of CD3<sup>+</sup> T cells in the spleen was decreased at day 60 (vs. control). Also, mRNA and protein expression of chemokines CCL21 and CCL19 and functional protein gp38 in spleen decreased significantly versus the control at day 60. In addition, ER-TR7 antigen protein expression was also decreased at day 60. Levels of T-helper (Th)1 cells significantly increased, whereas those of Th2 cells decreased significantly versus the control at day 60 in spleen. The finding revealed that <i>A. adenophora</i> could affect splenic immune function in rats by altering the fibroblast reticulocyte (FRC) network, as well as by causing an imbalance in Th1/Th2 cell ratios. This research provides new insights into potential mechanisms of spleen immunotoxicity due to exposures to <i>A. Adenophora.</i>
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