In vitro assessment of caseein phosphopeptide-amorphous calcium phosphate (CPP-ACP) in prevention of white spot lesions

• Main Authors: Zabokova-Bilbilova Efka, Sotirivska-Ivkovska Ana, Igić Marija, Mijoska Aneta, Stavreva Natasha, Spirov Vancho, Evrosimovska Biljana, Pecakov Kiril
• Format: Article
• Language: English
• Published: Medicinski fakultet i Klinika za stomatologiju, Niš 2019-01-01
• Series: Acta Stomatologica Naissi
• Subjects: dental caries; preventive treatment; ca and mg concentration
• Online Access: https://scindeks-clanci.ceon.rs/data/pdf/0352-5252/2019/0352-52521979909Z.pdf
• ISSN: 0352-5252; 1820-1202

Introduction: Brackets and various orthodontic elements that are used during the treatment, make the oral hygiene difficult and the accumulation in dental plaque much easier.Increasing the risk of dental caries during orthodontic treatment. The aim: of this study was to determine the concentration of Ca and Mg in artificial saliva after preventive treatment. Methods: The laboratory examination (in vitro) was performed in 90 healthy teeth extracted for orthodontic reasons. Three groups of thirty teeth were formed. The teeth from each group were separated in half in bucco-lingual direction. Thus, the control and test specimens were obtained from the same teeth. The brackets were bonded with GC Fuji OrthoTM LC (GC America Chicago, III), a resin-modified glass ionomer cement and the teeth then were stored in artificial saliva. Once per day, the teeth were coated with topical gel -CG Tooth Mousse in duration of 5 minutes and then returned to artificial saliva. Preventive treatment was at certain intervals of 1, 3 and 6 months. After each study period, the artificial saliva where the samples were stored was used for evaluation of Ca and Mg concentration by flame atomic absorption spectrophotometry. Results: The results of this study (in vitro) showed that the concentrations of Ca and Mg in artificial saliva were significantly increased even after the first month of application of this means which released Ca and other minerals (including fluoride free cement used to bond the brackets) with maximum value after a three-month application. These values were significantly lower after six months, probably as a result of its absorption into the enamel. The results for Mg are the same as for Ca, and the value is increased as well as its stability in saliva after the first month. Conclusions: The results of this in vitro study clearly indicate that preventive materials used in fixed orthodontic treatment inhibit the demineralization of enamel around brackets and orthodontic rings. Evidently, their use as additional preventive methods/tools, besides primary oral hygiene, is recommended.