Degradation of polycyclic aromatic hydrocarbons (phenanthrene and pyrene) by the ligninolytic fungi Ganoderma lucidum isolated from the hardwood stump

• Main Authors: Nikki Agrawal, Preeti Verma, Sushil Kumar Shahi
• Format: Article
• Language: English
• Published: SpringerOpen 2018-03-01
• Series: Bioresources and Bioprocessing
• Subjects: Ganoderma lucidum; Phenanthrene and pyrene degradation; Ligninolytic enzyme; HPLC; 18S rRNA sequencing
• Online Access: http://link.springer.com/article/10.1186/s40643-018-0197-5

Abstract Background Due to progress in science and technology, several harmful polycyclic aromatic hydrocarbons are synthesized and released into the environment. In the present investigation, a phenanthrene- and pyrene-degrading white rot fungi Ganoderma lucidum strain CCG1 was isolated from the Janjgir Champa district of Chhattisgarh, India, and then the degradation of phenanthrene and pyrene was estimated by high-performance liquid chromatography. Results It was found that G. lucidum able to degrade 99.65% of 20 mg/L of phenanthrene and 99.58% of pyrene in mineral salt broth after 30th day of incubation at 27 °C. G. lucidum produced significant amounts (p < 0.0001) of ligninolytic enzymes (Laccase, lignin peroxidase and manganese peroxidase) in the phenanthrene- and pyrene-containing mineral salt broth. G. lucidum produced maximum 10,788.00 U/L laccase, 3283.00 U/L Lignin peroxidase and 47,444.00 U/L Manganese peroxidase enzymes in the presence of phenanthrene and produced maximum 10,166.00 U/L laccase, 3613.00 U/L lignin peroxidase and 50,977.00 U/L manganese peroxidase enzymes in the presence of pyrene. Therefore, G. lucidum will be a potent phenanthrene and pyrene degrader from the environment.