Fluorescence-based fixative and vital staining of lipid droplets in Caenorhabditis elegans reveal fat stores using microscopy and flow cytometry approaches[S]

Fluorescence-based fixative and vital staining of lipid droplets in Caenorhabditis elegans reveal fat stores using microscopy and flow cytometry approaches[S]

The proportions of body fat and fat-free mass are determining factors of adiposity-associated diseases. Work in Caenorhabditis elegans has revealed evolutionarily conserved pathways of fat metabolism. Nevertheless, analysis of body composition and fat distribution in the nematodes has only been part...

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Bibliographic Details
Main Authors: Maja Klapper, Madeleine Ehmke, Daniela Palgunow, Mike Böhme, Christian Matthäus, Gero Bergner, Benjamin Dietzek, Jürgen Popp, Frank Döring
Format: Article
Language:English
Published: Elsevier 2011-06-01
Series:Journal of Lipid Research
Subjects:
C. elegans
CARS microscopy
fatty acid
fatty acid metabolism
fluorescence microscopy
obesity
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520313468

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http://www.sciencedirect.com/science/article/pii/S0022227520313468

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