Sequence and expression of a microspore cDNA clone with homology to a ribosomal protein

Sequence and expression of a microspore cDNA clone with homology to a ribosomal protein

A cDNA library was made to RNA from corn anthers containing developing pollen at the uninucleate microspore stage. A randomly selected clone from this library which contained an insert (531 bp) was isolated and sequenced. An open reading frame of 330 bp was located. Computer alignments of the putati...

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Main Authors: Michael P. Turcich, Amana Bokhari-Riza, Douglas A. Hamilton, Joseph P. Mascarenhas
Format: Article
Language:English
Published: Polish Botanical Society 2014-01-01
Series:Acta Societatis Botanicorum Poloniae
Subjects:
pollen
Zea mays
L7/L12 protein
ribosomes
Online Access:https://pbsociety.org.pl/journals/index.php/asbp/article/view/912
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spelling doaj-1e22f6ffabe74bfc95b6509cba45df202020-11-25T03:11:21ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802014-01-01651-2616310.5586/asbp.1996.011685Sequence and expression of a microspore cDNA clone with homology to a ribosomal proteinMichael P. Turcich0Amana Bokhari-Riza1Douglas A. Hamilton2Joseph P. Mascarenhas3State University of New YorkState University of New YorkState University of New YorkState University of New YorkA cDNA library was made to RNA from corn anthers containing developing pollen at the uninucleate microspore stage. A randomly selected clone from this library which contained an insert (531 bp) was isolated and sequenced. An open reading frame of 330 bp was located. Computer alignments of the putative amino acid sequence with sequences from GenBank and the SwissProt protein databases indicated homology to L12, an acidic ribosomal protein. RNA blot analysis showed highest levels of this mRNA in mature pollen. The significance of this observation in light of the known biochemistry of ribosome synthesis in developing pollen is discussed.https://pbsociety.org.pl/journals/index.php/asbp/article/view/912pollenZea maysL7/L12 proteinribosomes
collection DOAJ
language English
format Article
sources DOAJ
author Michael P. Turcich
Amana Bokhari-Riza
Douglas A. Hamilton
Joseph P. Mascarenhas
spellingShingle Michael P. Turcich
Amana Bokhari-Riza
Douglas A. Hamilton
Joseph P. Mascarenhas
Sequence and expression of a microspore cDNA clone with homology to a ribosomal protein
Acta Societatis Botanicorum Poloniae
pollen
Zea mays
L7/L12 protein
ribosomes
author_facet Michael P. Turcich
Amana Bokhari-Riza
Douglas A. Hamilton
Joseph P. Mascarenhas
author_sort Michael P. Turcich
title Sequence and expression of a microspore cDNA clone with homology to a ribosomal protein
title_short Sequence and expression of a microspore cDNA clone with homology to a ribosomal protein
title_full Sequence and expression of a microspore cDNA clone with homology to a ribosomal protein
title_fullStr Sequence and expression of a microspore cDNA clone with homology to a ribosomal protein
title_full_unstemmed Sequence and expression of a microspore cDNA clone with homology to a ribosomal protein
title_sort sequence and expression of a microspore cdna clone with homology to a ribosomal protein
publisher Polish Botanical Society
series Acta Societatis Botanicorum Poloniae
issn 2083-9480
publishDate 2014-01-01
description A cDNA library was made to RNA from corn anthers containing developing pollen at the uninucleate microspore stage. A randomly selected clone from this library which contained an insert (531 bp) was isolated and sequenced. An open reading frame of 330 bp was located. Computer alignments of the putative amino acid sequence with sequences from GenBank and the SwissProt protein databases indicated homology to L12, an acidic ribosomal protein. RNA blot analysis showed highest levels of this mRNA in mature pollen. The significance of this observation in light of the known biochemistry of ribosome synthesis in developing pollen is discussed.
topic pollen
Zea mays
L7/L12 protein
ribosomes
url https://pbsociety.org.pl/journals/index.php/asbp/article/view/912
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AT amanabokhaririza sequenceandexpressionofamicrosporecdnaclonewithhomologytoaribosomalprotein
AT douglasahamilton sequenceandexpressionofamicrosporecdnaclonewithhomologytoaribosomalprotein
AT josephpmascarenhas sequenceandexpressionofamicrosporecdnaclonewithhomologytoaribosomalprotein
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