Improvement of Ficin-Based Inhibitive Enzyme Assay for Toxic Metals Using Response Surface Methodology and Its Application for Near Real-Time Monitoring of Mercury in Marine Waters

Improvement of Ficin-Based Inhibitive Enzyme Assay for Toxic Metals Using Response Surface Methodology and Its Application for Near Real-Time Monitoring of Mercury in Marine Waters

Potentially toxic metals pollution in the Straits of Malacca warrants the development of rapid, simple and sensitive assays. Enzyme-based assays are excellent preliminary screening tools with near real-time potential. The heavy-metal assay based on the protease ficin was optimized for mercury detect...

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Bibliographic Details
Main Authors: Garba Uba, Motharasan Manogaran, Baskaran Gunasekaran, Mohd Izuan Effendi Halmi, Mohd Yunus Abd Shukor
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:International Journal of Environmental Research and Public Health
Subjects:
inhibitive assay
mercury
ficin
RSM
near real-time
Online Access:https://www.mdpi.com/1660-4601/17/22/8585

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