A reverse time-course method for transcriptional chase analyses of mRNA half-lives in cultured cells.

A reverse time-course method for transcriptional chase analyses of mRNA half-lives in cultured cells.

Standard methods for assessing mRNA stabilities in intact cells are labor-intensive and can generate half-life (t(1/2)) measures that are both imprecise and inaccurate. We describe modifications to a conventional tetracycline-conditional transcriptional chase method for analyzing mRNA stability that...

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Bibliographic Details
Main Authors: Osheiza Abdulmalik, Alyssa A Lombardi, J Eric Russell
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3396636?pdf=render

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