Morphological and pathogenic characterization of Fusarium oxysporum in lulo ( Solanum spp.)

Fusarium oxysporum is one of the most limiting fungal pathogens of lulo crop. To determine its pathogenicity, this work morphologically and pathogenically characterized F. oxysporum isolates from different lulo-growing municipalities of the Department of Nariño. Twenty isolates were evaluated throug...

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Main Authors: Yohana Patricia Anama, Ricardo Díaz, David Esteban Duarte-Alvarado, Tulio Cesar Lagos-Burbano
Format: Article
Language:English
Published: Universidad de Nariño 2021-06-01
Series:Revista de Ciencias Agrícolas
Subjects:
Online Access:https://revistas.udenar.edu.co/index.php/rfacia/article/view/5932
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spelling doaj-1dba44bced9747b4ac84b47454ea1b162021-09-20T13:50:39ZengUniversidad de NariñoRevista de Ciencias Agrícolas0120-01352256-22732021-06-013812037https://doi.org/10.22267/rcia.213801.142Morphological and pathogenic characterization of Fusarium oxysporum in lulo ( Solanum spp.)Yohana Patricia Anama0https://orcid.org/0000-0001-6948-8823Ricardo Díaz1https://orcid.org/0000-0002-5738-846XDavid Esteban Duarte-Alvarado2https://orcid.org/0000-0002-8896-3594Tulio Cesar Lagos-Burbano3https://orcid.org/0000-0001-9222-4674Universidad de NariñoUniversidad de NariñoUniversidad de NariñoUniversidad de NariñoFusarium oxysporum is one of the most limiting fungal pathogens of lulo crop. To determine its pathogenicity, this work morphologically and pathogenically characterized F. oxysporum isolates from different lulo-growing municipalities of the Department of Nariño. Twenty isolates were evaluated through a completely randomized design with two factors and three replicates per treatment, including a control. The first factor corresponded to 20 isolates of F. oxysporum and the second to 10 lulo genotypes. The morphological characterization involved determining growth rate (GR), color (CO), mycelial type (MT), medium coloration (Mc), shape (Sh), size (S), number (N) of macroconidial (Ma) and microconidial (Mi) septa, presence of chlamydospores (PC), and chlamydospore shape (CS). Moreover, the pathogenic characterization was based on the incubation period (IP), absolute growth rate (AGR), disease severity (DS), disease incidence (I), and vascular discoloration (VD). The morphological characterization demonstrated that all isolates corresponded to F. oxysporum. For IP, genotype G1 showed the lowest average at 18 days. For AGR, genotype G2 had the lowest height increase at 0.05 cm.day-1. For DS, genotype G1 reached the highest severity level (level 9) and a disease incidence of 100%. This study provides the first report of the special form of F. oxysporum f. sp. quitoense in Nariño. Solanum hirtum, Solanum sessiliflorum, and Solanum estramonifolium were resistant to the isolates evaluated, demonstrating that wild species should be considered as sources of resistance for breeding programs aiming to obtain resistant commercial genotypes.https://revistas.udenar.edu.co/index.php/rfacia/article/view/5932incidenceseveritysolanum hirtumsolanum sessiliflorumsolanum estramonifolium
collection DOAJ
language English
format Article
sources DOAJ
author Yohana Patricia Anama
Ricardo Díaz
David Esteban Duarte-Alvarado
Tulio Cesar Lagos-Burbano
spellingShingle Yohana Patricia Anama
Ricardo Díaz
David Esteban Duarte-Alvarado
Tulio Cesar Lagos-Burbano
Morphological and pathogenic characterization of Fusarium oxysporum in lulo ( Solanum spp.)
Revista de Ciencias Agrícolas
incidence
severity
solanum hirtum
solanum sessiliflorum
solanum estramonifolium
author_facet Yohana Patricia Anama
Ricardo Díaz
David Esteban Duarte-Alvarado
Tulio Cesar Lagos-Burbano
author_sort Yohana Patricia Anama
title Morphological and pathogenic characterization of Fusarium oxysporum in lulo ( Solanum spp.)
title_short Morphological and pathogenic characterization of Fusarium oxysporum in lulo ( Solanum spp.)
title_full Morphological and pathogenic characterization of Fusarium oxysporum in lulo ( Solanum spp.)
title_fullStr Morphological and pathogenic characterization of Fusarium oxysporum in lulo ( Solanum spp.)
title_full_unstemmed Morphological and pathogenic characterization of Fusarium oxysporum in lulo ( Solanum spp.)
title_sort morphological and pathogenic characterization of fusarium oxysporum in lulo ( solanum spp.)
publisher Universidad de Nariño
series Revista de Ciencias Agrícolas
issn 0120-0135
2256-2273
publishDate 2021-06-01
description Fusarium oxysporum is one of the most limiting fungal pathogens of lulo crop. To determine its pathogenicity, this work morphologically and pathogenically characterized F. oxysporum isolates from different lulo-growing municipalities of the Department of Nariño. Twenty isolates were evaluated through a completely randomized design with two factors and three replicates per treatment, including a control. The first factor corresponded to 20 isolates of F. oxysporum and the second to 10 lulo genotypes. The morphological characterization involved determining growth rate (GR), color (CO), mycelial type (MT), medium coloration (Mc), shape (Sh), size (S), number (N) of macroconidial (Ma) and microconidial (Mi) septa, presence of chlamydospores (PC), and chlamydospore shape (CS). Moreover, the pathogenic characterization was based on the incubation period (IP), absolute growth rate (AGR), disease severity (DS), disease incidence (I), and vascular discoloration (VD). The morphological characterization demonstrated that all isolates corresponded to F. oxysporum. For IP, genotype G1 showed the lowest average at 18 days. For AGR, genotype G2 had the lowest height increase at 0.05 cm.day-1. For DS, genotype G1 reached the highest severity level (level 9) and a disease incidence of 100%. This study provides the first report of the special form of F. oxysporum f. sp. quitoense in Nariño. Solanum hirtum, Solanum sessiliflorum, and Solanum estramonifolium were resistant to the isolates evaluated, demonstrating that wild species should be considered as sources of resistance for breeding programs aiming to obtain resistant commercial genotypes.
topic incidence
severity
solanum hirtum
solanum sessiliflorum
solanum estramonifolium
url https://revistas.udenar.edu.co/index.php/rfacia/article/view/5932
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