The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle

We used a rapid and simple protocol using lysolecithin for mapping HS sites in vivo. The protocol is based on partial digestion with DNase I of exponentially growing cells following permeabilization by short treatment with lysolecithin. Using this protocol, we analyzed the chromatin structure of the...

Full description

Bibliographic Details
Main Authors: KATRIN ZIMMERMANN, MARLIS HOLTZ, LOC PHI-VAN
Format: Article
Language:English
Published: BMC 2007-01-01
Series:Biological Research
Subjects:
Online Access:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200009
Description
Summary:We used a rapid and simple protocol using lysolecithin for mapping HS sites in vivo. The protocol is based on partial digestion with DNase I of exponentially growing cells following permeabilization by short treatment with lysolecithin. Using this protocol, we analyzed the chromatin structure of the region surrounding two overlapping elements, an origin of bidirectional DNA replication and the GAS41 promoter, in chicken myelomonocytic HD11 cells arrested in G0, G0 and S phases as well as at the G0/S border. The results show that the chromatin of this region became more nuclease sensitive when cells were arrested in G0 phase and that this change in chromatin structure was reversible after the cells began to enter S phase
ISSN:0716-9760
0717-6287