Expression and phosphorylation state analysis of intracellular protein kinases using Multi-PK antibody and Phos-tag SDS-PAGE

Expression and phosphorylation state analysis of intracellular protein kinases using Multi-PK antibody and Phos-tag SDS-PAGE

Protein kinase expression and activity play important roles in diverse cellular functions through regulation of phosphorylation signaling. The most commonly used tools for detecting the protein kinase are protein kinase-specific antibodies, and phosphorylation site-specific antibodies were used for...

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Bibliographic Details
Main Authors: Yasunori Sugiyama, Syouichi Katayama, Isamu Kameshita, Keiko Morisawa, Takuma Higuchi, Hiroshi Todaka, Eiji Kinoshita, Emiko Kinoshita-Kikuta, Tohru Koike, Taketoshi Taniguchi, Shuji Sakamoto
Format: Article
Language:English
Published: Elsevier 2015-01-01
Series:MethodsX
Subjects:
Multi-PK antibody
Phos-tag
Protein kinase
Phosphorylation Signaling
Kinome
Online Access:http://www.sciencedirect.com/science/article/pii/S221501611500062X

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