Hydrodynamics-Based Transplacental Delivery as a Useful Noninvasive Tool for Manipulating Fetal Genome

Hydrodynamics-Based Transplacental Delivery as a Useful Noninvasive Tool for Manipulating Fetal Genome

We previously demonstrated that the injection of pregnant wild-type female mice (carrying enhanced green fluorescent protein (EGFP)-expressing transgenic fetuses) at embryonic day (E) 12.5 with an all-in-one plasmid conferring the expression of both Cas9 and guide RNA (targeted to the <i>EGFP&...

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Bibliographic Details
Main Authors: Shingo Nakamura, Naoko Ando, Satoshi Watanabe, Eri Akasaka, Masayuki Ishihara, Masahiro Sato
Format: Article
Language:English
Published: MDPI AG 2020-07-01
Series:Cells
Subjects:
cardiomyocytes
CRISPR/Cas9
fetal gene therapy
fetuses
genome editing
hydrodynamics-based gene delivery (HGD) system
Online Access:https://www.mdpi.com/2073-4409/9/7/1744

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