Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin

Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin

Summary: Current knowledge on brain oligodendrogenesis, composed of three sequential waves from different regions, is mainly obtained from rodent studies. Oligodendroglial development and myelination in the rodent brain may not fully mirror those processes in the human brain. Here, we provide a step...

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Main Authors: Hyosung Kim, Peng Jiang
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Neuroscience
Stem Cells
Cell Differentiation
Organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721001507
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spelling doaj-1cde6547581145a68eb35a52edb02d9a2021-06-21T04:25:10ZengElsevierSTAR Protocols2666-16672021-06-0122100443Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelinHyosung Kim0Peng Jiang1Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN 37212, USA; Corresponding authorDepartment of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ 08854, USA; Corresponding authorSummary: Current knowledge on brain oligodendrogenesis, composed of three sequential waves from different regions, is mainly obtained from rodent studies. Oligodendroglial development and myelination in the rodent brain may not fully mirror those processes in the human brain. Here, we provide a step-by-step protocol for generating fused forebrain organoids derived from human pluripotent stem cells. These fused organoids recapitulate human oligodendroglial developments, offering innovative insights into human myelination research in vitro.For complete details on the use and execution of this protocol, please refer to Cameron-Curry and Le Douarin (1995), Kessaris et al. (2006), and Kim et al. (2019).http://www.sciencedirect.com/science/article/pii/S2666166721001507NeuroscienceStem CellsCell DifferentiationOrganoids
collection DOAJ
language English
format Article
sources DOAJ
author Hyosung Kim
Peng Jiang
spellingShingle Hyosung Kim
Peng Jiang
Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin
STAR Protocols
Neuroscience
Stem Cells
Cell Differentiation
Organoids
author_facet Hyosung Kim
Peng Jiang
author_sort Hyosung Kim
title Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin
title_short Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin
title_full Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin
title_fullStr Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin
title_full_unstemmed Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin
title_sort generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2021-06-01
description Summary: Current knowledge on brain oligodendrogenesis, composed of three sequential waves from different regions, is mainly obtained from rodent studies. Oligodendroglial development and myelination in the rodent brain may not fully mirror those processes in the human brain. Here, we provide a step-by-step protocol for generating fused forebrain organoids derived from human pluripotent stem cells. These fused organoids recapitulate human oligodendroglial developments, offering innovative insights into human myelination research in vitro.For complete details on the use and execution of this protocol, please refer to Cameron-Curry and Le Douarin (1995), Kessaris et al. (2006), and Kim et al. (2019).
topic Neuroscience
Stem Cells
Cell Differentiation
Organoids
url http://www.sciencedirect.com/science/article/pii/S2666166721001507
work_keys_str_mv AT hyosungkim generationofhumanpluripotentstemcellderivedfusedorganoidswitholigodendrogliaandmyelin
AT pengjiang generationofhumanpluripotentstemcellderivedfusedorganoidswitholigodendrogliaandmyelin
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