A Simple and Efficient Genetic Immunization Protocol for the Production of Highly Specific Polyclonal and Monoclonal Antibodies against the Native Form of Mammalian Proteins

A Simple and Efficient Genetic Immunization Protocol for the Production of Highly Specific Polyclonal and Monoclonal Antibodies against the Native Form of Mammalian Proteins

We have generated polyclonal and monoclonal antibodies by genetic immunization over the last two decades. In this paper, we present our most successful methodology acquired over these years and present the animals in which we obtained the highest rates of success. The technique presented is convenie...

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Bibliographic Details
Main Authors: Julie Pelletier, Hervé Agonsanou, Fabiana Manica, Elise G. Lavoie, Mabrouka Salem, Patrick Luyindula, Romuald Brice Babou Kammoe, Jean Sévigny
Format: Article
Language:English
Published: MDPI AG 2020-09-01
Series:International Journal of Molecular Sciences
Subjects:
immunization
antibody
protocol
guinea pig
cDNA
Online Access:https://www.mdpi.com/1422-0067/21/19/7074

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