Multiplex single-cell visualization of nucleic acids and protein during HIV infection

Multiplex single-cell visualization of nucleic acids and protein during HIV infection

Technical limitations in simultaneous microscopic visualization of HIV transcription from individual integration sites have curtailed progress in the field. Here the authors report a branched DNA in situ hybridization method for direct single-cell visualization of HIV DNA, RNA, and protein.

Bibliographic Details
Main Authors: Maritza Puray-Chavez, Philip R. Tedbury, Andrew D. Huber, Obiaara B. Ukah, Vincent Yapo, Dandan Liu, Juan Ji, Jennifer J. Wolf, Alan N. Engelman, Stefan G. Sarafianos
Format: Article
Language:English
Published: Nature Publishing Group 2017-12-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-017-01693-z
Description
Summary:Technical limitations in simultaneous microscopic visualization of HIV transcription from individual integration sites have curtailed progress in the field. Here the authors report a branched DNA in situ hybridization method for direct single-cell visualization of HIV DNA, RNA, and protein.
ISSN:2041-1723

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