Optimized DPPH assay in a detergent-based buffer system for measuring antioxidant activity of proteins

Optimized DPPH assay in a detergent-based buffer system for measuring antioxidant activity of proteins

The free radical method using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) is a well established assay for the in vitro determination of antioxidant activity in food and biological extracts. The standard DPPH assay uses methanol or ethanol as solvents, or buffered alcoholic solutions in a ratio of 40%/60%...

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Bibliographic Details
Main Authors:	Sascha C.T. Nicklisch, J. Herbert Waite
Format:	Article
Language:	English
Published:	Elsevier 2014-01-01
Series:	MethodsX
Subjects:	DPPH radical Antioxidant proteins Non-ionic detergent pH range
Online Access:	http://www.sciencedirect.com/science/article/pii/S2215016114000181

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