3D-Printed, Pocket-Size Diffusion Cells for Skin Permeation Investigation

3D-Printed, Pocket-Size Diffusion Cells for Skin Permeation Investigation

Here we present a novel, compact 3D-printed diffusion cell as an <i>in vitro </i>tool for skin permeation investigation. As proof-of-concept, a diffusion cell for studying the permeation of a model molecule (FITC-dextran, 4 kDa) through explanted mice skin is fabricated and characterized...

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Main Authors: Giulia Suarato, Raffaele Spanò, Rosalia Bertorelli, Alberto Diaspro, Athanassia Athanassiou, Salvatore Surdo
Format: Article
Language:English
Published: MDPI AG 2018-12-01
Series:Proceedings
Subjects:
transdermal drug-delivery
molecule diffusivity
bottom-up approach
lab-on-chip
Online Access:https://www.mdpi.com/2504-3900/2/13/945
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spelling doaj-1b6cd66b36f24cdba2c1b5b1547654b72020-11-24T22:18:47ZengMDPI AGProceedings2504-39002018-12-0121394510.3390/proceedings2130945proceedings21309453D-Printed, Pocket-Size Diffusion Cells for Skin Permeation InvestigationGiulia Suarato0Raffaele Spanò1Rosalia Bertorelli2Alberto Diaspro3Athanassia Athanassiou4Salvatore Surdo5Smart Materials, Istituto Italiano di Tecnologia, 16163 Genova, ItalyD3 PharmaChemistry, Istituto Italiano di Tecnologia, 16163 Genova, ItalyIn vivo Pharmacology Facility, Istituto Italiano di Tecnologia, 16163 Genova, ItalyNanophysics, Istituto Italiano di Tecnologia, 16163 Genova, ItalySmart Materials, Istituto Italiano di Tecnologia, 16163 Genova, ItalyNanophysics, Istituto Italiano di Tecnologia, 16163 Genova, ItalyHere we present a novel, compact 3D-printed diffusion cell as an <i>in vitro </i>tool for skin permeation investigation. As proof-of-concept, a diffusion cell for studying the permeation of a model molecule (FITC-dextran, 4 kDa) through explanted mice skin is fabricated and characterized. Good viability of the tissue up to 24 h incubation in the cell is demonstrated via MTT assays. The real-time diffusion of the molecule by means of fluorescence microscopy allowed the determination of its diffusivity through the skin (~2&#183;10<sup>&#8722;10</sup> m<sup>2</sup>/s). Our results open the door for the real-time, high-throughput and cost-effective investigation of skin in any labs.https://www.mdpi.com/2504-3900/2/13/945transdermal drug-deliverymolecule diffusivitybottom-up approachlab-on-chip
collection DOAJ
language English
format Article
sources DOAJ
author Giulia Suarato
Raffaele Spanò
Rosalia Bertorelli
Alberto Diaspro
Athanassia Athanassiou
Salvatore Surdo
spellingShingle Giulia Suarato
Raffaele Spanò
Rosalia Bertorelli
Alberto Diaspro
Athanassia Athanassiou
Salvatore Surdo
3D-Printed, Pocket-Size Diffusion Cells for Skin Permeation Investigation
Proceedings
transdermal drug-delivery
molecule diffusivity
bottom-up approach
lab-on-chip
author_facet Giulia Suarato
Raffaele Spanò
Rosalia Bertorelli
Alberto Diaspro
Athanassia Athanassiou
Salvatore Surdo
author_sort Giulia Suarato
title 3D-Printed, Pocket-Size Diffusion Cells for Skin Permeation Investigation
title_short 3D-Printed, Pocket-Size Diffusion Cells for Skin Permeation Investigation
title_full 3D-Printed, Pocket-Size Diffusion Cells for Skin Permeation Investigation
title_fullStr 3D-Printed, Pocket-Size Diffusion Cells for Skin Permeation Investigation
title_full_unstemmed 3D-Printed, Pocket-Size Diffusion Cells for Skin Permeation Investigation
title_sort 3d-printed, pocket-size diffusion cells for skin permeation investigation
publisher MDPI AG
series Proceedings
issn 2504-3900
publishDate 2018-12-01
description Here we present a novel, compact 3D-printed diffusion cell as an <i>in vitro </i>tool for skin permeation investigation. As proof-of-concept, a diffusion cell for studying the permeation of a model molecule (FITC-dextran, 4 kDa) through explanted mice skin is fabricated and characterized. Good viability of the tissue up to 24 h incubation in the cell is demonstrated via MTT assays. The real-time diffusion of the molecule by means of fluorescence microscopy allowed the determination of its diffusivity through the skin (~2&#183;10<sup>&#8722;10</sup> m<sup>2</sup>/s). Our results open the door for the real-time, high-throughput and cost-effective investigation of skin in any labs.
topic transdermal drug-delivery
molecule diffusivity
bottom-up approach
lab-on-chip
url https://www.mdpi.com/2504-3900/2/13/945
work_keys_str_mv AT giuliasuarato 3dprintedpocketsizediffusioncellsforskinpermeationinvestigation
AT raffaelespano 3dprintedpocketsizediffusioncellsforskinpermeationinvestigation
AT rosaliabertorelli 3dprintedpocketsizediffusioncellsforskinpermeationinvestigation
AT albertodiaspro 3dprintedpocketsizediffusioncellsforskinpermeationinvestigation
AT athanassiaathanassiou 3dprintedpocketsizediffusioncellsforskinpermeationinvestigation
AT salvatoresurdo 3dprintedpocketsizediffusioncellsforskinpermeationinvestigation
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