Metabolizable Protein: 1. Predicting Equations to Estimate Microbial Crude Protein Synthesis in Small Ruminants

• Main Authors: Stefanie Alvarenga Santos, Gleidson Giordano Pinto de Carvalho, José Augusto Gomes Azevêdo, Diego Zanetti, Edson Mauro Santos, Mara Lucia Albuquerque Pereira, Elzania Sales Pereira, Aureliano José Vieira Pires, Sebastião de Campos Valadares Filho, Izabelle Auxiliadora Molina de Almeida Teixeira, Manuela Silva Libânio Tosto, Laudi Cunha Leite, Lays Débora Silva Mariz
• Format: Article
• Language: English
• Published: Frontiers Media S.A. 2021-06-01
• Series: Frontiers in Veterinary Science
• Subjects: bacteria; goat; microorganisms; sheep; rumen; yield
• Online Access: https://www.frontiersin.org/articles/10.3389/fvets.2021.650248/full

Microbial crude protein (MCP) produced in rumen could be estimated by a variety of protocols of experimental sampling and analysis. However, a model to estimate this value is necessary when protein requirements are calculated for small ruminants. This model could be useful to calculate rumen degradable protein (RDP) requirements from metabolizable protein (MP). Then, our objective was to investigate if there is a difference in MCP efficiency between sheep and goats, and to fit equations to predict ruminal MCP production from dietary energy intake. The database consisted of 19 studies with goats (n = 176) and sheep (n = 316), and the variables MCP synthesis (g/day), total digestible nutrients (TDN), and organic matter (OM) intakes (g/day), and OM digestibility (g/kg DM) were registered for both species. The database was used for two different purposes, where 70% of the values were sorted to fit equations, and 30% for validation. A meta-analytical procedure was carried out using the MIXED procedure of SAS, specie was considered as the fixed dummy effect, and the intercept and slope nested in the study were considered random effects. No effect of specie was observed for the estimation of MCP from TDN, digestible Organic Matter (dOM), or metabolizable energy (ME) intakes (P > 0.05), considering an equation with or without an intercept. Therefore, single models including both species at the same fitting were validated. The following equations MCP (g/day) = 12.7311 + 59.2956 × TDN intake (AIC = 3,004.6); MCP (g/day) = 15.7764 + 62.2612 × dOM intake (AIC = 2,755.1); and MCP (g/day) = 12.7311 + 15.3000 × ME intake (AIC = 3,007.3) presented lower values for the mean square error of prediction (MSEP) and its decomposition, and similar values for the concordance correlation coefficient (CCC) and for the residual mean square error (RMSE) when compared with equations fitted without an intercept. The intercept and slope pooled test was significant for equations without an intercept (P < 0.05), indicating that observed and predicted data differed. In contrast, predicted and observed data for complete equations were similar (P > 0.05).