The Optimized Workflow for Sample Preparation in LC-MS/MS-Based Urine Proteomics

The Optimized Workflow for Sample Preparation in LC-MS/MS-Based Urine Proteomics

The sample condition is an important factor in urine proteomics with stability and accuracy. However, a general protocol of urine protein preparation in mass spectrometry analysis has not yet been established. Here, we proposed a workflow for optimized sample preparation based on methanol/chloroform...

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Bibliographic Details
Main Authors: Suguru Saito, Yoshitoshi Hirao, Ali F. Quadery, Bo Xu, Amr Elguoshy, Hidehiko Fujinaka, Shohei Koma, Keiko Yamamoto, Tadashi Yamamoto
Format: Article
Language:English
Published: MDPI AG 2019-06-01
Series:Methods and Protocols
Subjects:
Urine proteomics
Urine protein precipitation
Urine biomarker
Online Access:https://www.mdpi.com/2409-9279/2/2/46

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