Cytochemical examination of blood cells of children with chronic tonsillitis and type 1 diabetes mellitus
Aim of the work: to study the state of the enzyme activity of the energy exchange of succinate dehydrogenase (SDH) and hydrolytic enzyme acid phosphatase (AF) in neutrophils and lymphocytes on the basis of their cytochemical study in blood cells of children with CT in combination with DM 1. Materia...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
PC Technology Center
2018-03-01
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| Series: | ScienceRise: Medical Science |
| Subjects: | |
| Online Access: | http://journals.uran.ua/sr_med/article/view/127424 |
| Summary: | Aim of the work: to study the state of the enzyme activity of the energy exchange of succinate dehydrogenase (SDH) and hydrolytic enzyme acid phosphatase (AF) in neutrophils and lymphocytes on the basis of their cytochemical study in blood cells of children with CT in combination with DM 1.
Materials and methods: the blood of 41 children aged from 6 to 12 years was examined. All patients were divided into 2 clinical groups: the first (main) group included 30 children, who were diagnosed with CT in combination with DM 1, and the second (control group of comparison) - 11 children with CT without DM 1. Blood smears were examined under a light microscope and an oil immersion. The results were evaluated by a semi-quantitative method, with the calculation of the average cytochemical coefficient (ACC)
Results: a significant decrease in SDH was observed in the group of patients with CT in combination with DM 1, and ACC was respectively, for neutrophils, 0.85 ± 0.22 and 1.27 ± 0.31 for lymphocytes, as compared with patients with only CT for neutrophils 0 , 37 ± 0.18 (p <0.05), and 0.53 ± 0.12 (p <0.05) for lymphocytes. The combination of DM 1 and CT reduces the activity of the lysosomal enzyme acid phosphatase. At the same time, the ACC of AF with a combined pathology is 0.286 ± 0.07 for neutrophils and 0.165 ± 0.055 for lymphocytes, and 0.361 ± 0.057 (p <0.05) and 0.213 ± 0.038 (p <0.05), respectively, for comparison with CT.
Conclusions: the revealed changes in the cytochemical characteristics of blood cells reliably determine the nature of metabolic disorders with DM 1 in children and affect the course of CT. Violation of the activity of the enzymes of energy supply and hydrolysis in neutrophils and lymphocytes in patients indicates a decrease in the functional activity of these cell elements, which may affect their bactericidal and immunological protection in patients with DM 1 and CT, and also contribute to a more severe course of CT and frequent exacerbations of chronic diseases of lymphoepithelial pharyngeal formations |
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| ISSN: | 2519-478X 2519-4798 |