Modified CTAB protocol for RNA extraction from Lemon balm (Melissa officinalis L.)

Modified CTAB protocol for RNA extraction from Lemon balm (Melissa officinalis L.)

<p>Ribonucleic acid (RNA) quality and integrity are crucial for many studies in plant molecular biology. High-quality RNA extraction from plants with high levels of compounds such as polysaccharides, polyphenols, and other secondary metabolites are problematic. RNA extraction from Lemon balm t...

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Main Authors: Fatemeh RAHMANI, Leila AMRAEE
Format: Article
Language:English
Published: University of Ljubljana, Biotechnical Faculty 2020-02-01
Series:Acta Agriculturae Slovenica
Subjects:
melissa officinalis
ctab
rna
rt-pcr
Online Access:http://ojs.aas.bf.uni-lj.si/index.php/AAS/article/view/692
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spelling doaj-1ad56f60894648a493835c01d91e75702020-11-25T00:36:19ZengUniversity of Ljubljana, Biotechnical FacultyActa Agriculturae Slovenica1581-91751854-19412020-02-011151535710.14720/aas.2020.115.1.692347Modified CTAB protocol for RNA extraction from Lemon balm (Melissa officinalis L.)Fatemeh RAHMANI0Leila AMRAEE1Department of Biology, Faculty of Sciences, Urmia University, Urmia, IranDepartment of Biology, Faculty of Sciences, Urmia University, Urmia, Iran<p>Ribonucleic acid (RNA) quality and integrity are crucial for many studies in plant molecular biology. High-quality RNA extraction from plants with high levels of compounds such as polysaccharides, polyphenols, and other secondary metabolites are problematic. RNA extraction from Lemon balm tissues can be difficult due to the presence of polyphenolic and polysaccharide compounds or can be done by expensive protocols. This study shows improvement of a CTAB-based protocol which allows rapid and easy isolation of high-quality RNA from Lemon balm plant. The RNA obtained is suitable for cDNA synthesis and RT-PCR experiments.</p>http://ojs.aas.bf.uni-lj.si/index.php/AAS/article/view/692melissa officinalisctabrnart-pcr
collection DOAJ
language English
format Article
sources DOAJ
author Fatemeh RAHMANI
Leila AMRAEE
spellingShingle Fatemeh RAHMANI
Leila AMRAEE
Modified CTAB protocol for RNA extraction from Lemon balm (Melissa officinalis L.)
Acta Agriculturae Slovenica
melissa officinalis
ctab
rna
rt-pcr
author_facet Fatemeh RAHMANI
Leila AMRAEE
author_sort Fatemeh RAHMANI
title Modified CTAB protocol for RNA extraction from Lemon balm (Melissa officinalis L.)
title_short Modified CTAB protocol for RNA extraction from Lemon balm (Melissa officinalis L.)
title_full Modified CTAB protocol for RNA extraction from Lemon balm (Melissa officinalis L.)
title_fullStr Modified CTAB protocol for RNA extraction from Lemon balm (Melissa officinalis L.)
title_full_unstemmed Modified CTAB protocol for RNA extraction from Lemon balm (Melissa officinalis L.)
title_sort modified ctab protocol for rna extraction from lemon balm (melissa officinalis l.)
publisher University of Ljubljana, Biotechnical Faculty
series Acta Agriculturae Slovenica
issn 1581-9175
1854-1941
publishDate 2020-02-01
description <p>Ribonucleic acid (RNA) quality and integrity are crucial for many studies in plant molecular biology. High-quality RNA extraction from plants with high levels of compounds such as polysaccharides, polyphenols, and other secondary metabolites are problematic. RNA extraction from Lemon balm tissues can be difficult due to the presence of polyphenolic and polysaccharide compounds or can be done by expensive protocols. This study shows improvement of a CTAB-based protocol which allows rapid and easy isolation of high-quality RNA from Lemon balm plant. The RNA obtained is suitable for cDNA synthesis and RT-PCR experiments.</p>
topic melissa officinalis
ctab
rna
rt-pcr
url http://ojs.aas.bf.uni-lj.si/index.php/AAS/article/view/692
work_keys_str_mv AT fatemehrahmani modifiedctabprotocolforrnaextractionfromlemonbalmmelissaofficinalisl
AT leilaamraee modifiedctabprotocolforrnaextractionfromlemonbalmmelissaofficinalisl
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