A Glucose Sensor Based on Glucose Oxidase Immobilized by Electrospinning Nanofibrous Polymer Membranes Modified with Carbon Nanotubes

• Main Authors: You Wang, Hui Xu, Zhengang Wang, Ruifen Hu, Zhiyuan Luo, Zhikang Xu, Guang Li
• Format: Article
• Language: English
• Published: IFSA Publishing, S.L. 2013-05-01
• Series: Sensors & Transducers
• Subjects: Electrospinning; Glucose biosensor; Enzyme immobilization; Carbon nanotubes
• Online Access: http://www.sensorsportal.com/HTML/DIGEST/may_2013/P_1210.pdf
• ISSN: 2306-8515; 1726-5479

A glucose biosensor based on glucose oxidase immobilized by electrospinning nanofibrous membranes has been developed. Nanofibrous membranes were electrospun from the solution of poly(acrylonitrile-co-acrylic acid) containing carbon nanotubes suspension and directly deposited on Pt electrodes for immobilizing glucose oxidase. The morphologies and structure of the nanofibrous membranes with or without carbon nanotubes were characterized by scanning electron microscopy. The fabrication parameters of nanofibers were optimized such as thickness of the nanofibrous membranes and mass ration of carbon nanotubes. The biosensor showed the relationship with a concentration range of 0.1–10 mM and response time was 60 s. The sensitivity of carbon nanotubes modified biosensors was two times larger than which of no carbon nanotubes modified ones. The pH effect, interference and lifetime of biosensors were discussed.