High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries

High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries

Average Nucleotide Identity (ANI) is a robust and useful measure to gauge genetic relatedness between two genomes. Here, the authors develop FastANI, a method to compute ANI using alignment-free approximate sequence mapping, and show 95% ANI is an accurate threshold for demarcating prokaryotic speci...

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Main Authors: Chirag Jain, Luis M. Rodriguez-R, Adam M. Phillippy, Konstantinos T. Konstantinidis, Srinivas Aluru
Format: Article
Language:English
Published: Nature Publishing Group 2018-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-07641-9
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spelling doaj-1a91648286dc4ad7b362fb1f3a14aece2021-05-11T09:57:45ZengNature Publishing GroupNature Communications2041-17232018-11-01911810.1038/s41467-018-07641-9High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundariesChirag Jain0Luis M. Rodriguez-R1Adam M. Phillippy2Konstantinos T. Konstantinidis3Srinivas Aluru4School of Computational Science and Engineering, Georgia Institute of TechnologySchool of Civil and Environmental Engineering, Georgia Institute of TechnologyNational Human Genome Research Institute, National Institutes of HealthSchool of Civil and Environmental Engineering, Georgia Institute of TechnologySchool of Computational Science and Engineering, Georgia Institute of TechnologyAverage Nucleotide Identity (ANI) is a robust and useful measure to gauge genetic relatedness between two genomes. Here, the authors develop FastANI, a method to compute ANI using alignment-free approximate sequence mapping, and show 95% ANI is an accurate threshold for demarcating prokaryotic species by analyzing about 90,000 prokaryotic genomes.https://doi.org/10.1038/s41467-018-07641-9
collection DOAJ
language English
format Article
sources DOAJ
author Chirag Jain
Luis M. Rodriguez-R
Adam M. Phillippy
Konstantinos T. Konstantinidis
Srinivas Aluru
spellingShingle Chirag Jain
Luis M. Rodriguez-R
Adam M. Phillippy
Konstantinos T. Konstantinidis
Srinivas Aluru
High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries
Nature Communications
author_facet Chirag Jain
Luis M. Rodriguez-R
Adam M. Phillippy
Konstantinos T. Konstantinidis
Srinivas Aluru
author_sort Chirag Jain
title High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries
title_short High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries
title_full High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries
title_fullStr High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries
title_full_unstemmed High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries
title_sort high throughput ani analysis of 90k prokaryotic genomes reveals clear species boundaries
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2018-11-01
description Average Nucleotide Identity (ANI) is a robust and useful measure to gauge genetic relatedness between two genomes. Here, the authors develop FastANI, a method to compute ANI using alignment-free approximate sequence mapping, and show 95% ANI is an accurate threshold for demarcating prokaryotic species by analyzing about 90,000 prokaryotic genomes.
url https://doi.org/10.1038/s41467-018-07641-9
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AT konstantinostkonstantinidis highthroughputanianalysisof90kprokaryoticgenomesrevealsclearspeciesboundaries
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