Role of Stomatal Conductance in Modifying the Dose Response of Stress-Volatile Emissions in Methyl Jasmonate Treated Leaves of Cucumber (<i>Cucumis Sativa</i>)

• Main Authors: Yifan Jiang, Jiayan Ye, Bahtijor Rasulov, Ülo Niinemets
• Format: Article
• Language: English
• Published: MDPI AG 2020-02-01
• Series: International Journal of Molecular Sciences
• Subjects: stomatal closure; acute emission; lox compounds; methanol; photosynthesis; meja; abiotic stress interaction
• Online Access: https://www.mdpi.com/1422-0067/21/3/1018

Treatment by volatile plant hormone methyl jasmonate (MeJA) leads to release of methanol and volatiles of lipoxygenase pathway (LOX volatiles) in a dose-dependent manner, but how the dose dependence is affected by stomatal openness is poorly known. We studied the rapid (0&#8722;60 min after treatment) response of stomatal conductance (<i>G</i>_s), net assimilation rate (<i>A</i>), and LOX and methanol emissions to varying MeJA concentrations (0.2&#8722;50 mM) in cucumber (<i>Cucumis sativus</i>) leaves with partly open stomata and in leaves with reduced <i>G</i>_s due to drought and darkness. Exposure to MeJA led to initial opening of stomata due to an osmotic shock, followed by MeJA concentration-dependent reduction in <i>G</i>_s, whereas <i>A</i> initially decreased, followed by recovery for lower MeJA concentrations and time-dependent decline for higher MeJA concentrations. Methanol and LOX emissions were elicited in a MeJA concentration-dependent manner, whereas the peak methanol emissions (15&#8722;20 min after MeJA application) preceded LOX emissions (20&#8722;60 min after application). Furthermore, peak methanol emissions occurred earlier in treatments with higher MeJA concentration, while the opposite was observed for LOX emissions. This difference reflected the circumstance where the rise of methanol release partly coincided with MeJA-dependent stomatal opening, while stronger stomatal closure at higher MeJA concentrations progressively delayed peak LOX emissions. We further observed that drought-dependent reduction in <i>G</i>_s ameliorated MeJA effects on foliage physiological characteristics, underscoring that MeJA primarily penetrates through the stomata. However, despite reduced <i>G</i>_s, dark pretreatment amplified stress-volatile release upon MeJA treatment, suggesting that increased leaf oxidative status due to sudden illumination can potentiate the MeJA response. Taken together, these results collectively demonstrate that the MeJA dose response of volatile emission is controlled by stomata that alter MeJA uptake and volatile release kinetics and by leaf oxidative status in a complex manner.