BACTERIAL EXPRESSION OPTIMIZATION OF HUMAN AIMP1/Р43 RECOMBINANT PROTEIN – A COMPONENT OF HUMAN MULTISYNTHETASE COMPLEX IN STRAIN OF ESCHERICHIA COLI BL21(DE3)RIL
Aim. The optimization of conditions for bacterial expression of recombinant protein AIMP1/p43 has been carried out in order to increase the yield of stable protein in solution. Methods. Protein expression in bacterial expression system, metal-chelating affinity chromatography, gel-electrophoresis. R...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Odessa I. I. Mechnikov National University
2015-12-01
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| Series: | Mìkrobìologìâ ì Bìotehnologìâ |
| Subjects: | |
| Online Access: | http://mbt.onu.edu.ua/article/view/57455 |